Systems Properties and Spatiotemporal Regulation of Cell Position Variability during Embryogenesis
Systems Properties and Spatiotemporal Regulation of Cell Position Variability during Embryogenesis
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胚胎发生过程中细胞位置变异的系统特性和时空调节
DOI：
10.1016/j.celrep.2018.12.052
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发表时间：
2019-01-08
期刊：
影响因子：
8.8
通讯作者：
Du, Zhuo
中科院分区：
文献类型：
--
作者：
Li, Xiaoyu;Zhao, Zhiguang;Du, Zhuo
发育生物学中一个有趣的问题是发育过程是如何在个体间实现高可重复性的？对表型变异中包含的信息进行深入分析，为解决这一问题提供了一个重要的视角。在这项工作中，我们提出了秀丽隐杆线虫胚胎发生过程中细胞位置变异的定量和功能分析。我们发现细胞位置变化是高度确定性的，并受到内在和外在机制的调节。位置变异是由细胞谱系特性决定的，并与细胞的不同发育特性相关联，包括胚胎定位、细胞接触和左右对称。细胞位置变异性的时间动态是高度一致的，命运规范有助于在系统范围内减少变异性，从而提供缓冲策略。位置变异性在整个胚胎发生过程中受到严格调节，细胞-细胞连接功能限制了变异性。我们的研究结果提供了对发育过程中细胞变异性的系统特性和时空控制的见解。
An intriguing question in developmental biology is how do developmental processes achieve high reproducibility among individuals? An in-depth analysis of information contained in phenotypic variability provides an important perspective to address this question. In this work, we present a quantitative and functional analysis of cell position variability during Caenorhabditis elegans embryogenesis. We find that cell position variability is highly deterministic and regulated by intrinsic and extrinsic mechanisms. Positional variability is determined by cell lineage identity and is coupled to diverse developmental properties of cells, including embryonic localization, cell contact, and left-right symmetry. Temporal dynamics of cell position variability are highly concordant, and fate specification contributes to a systems-wide reduction of variability that could provide a buffering strategy. Positional variability is stringently regulated throughout embryogenesis and cell-cell junctions function to restrict variability. Our results provide insight into systems properties and spatiotemporal control of cellular variability during development.