Intraocular pressure measurement in mice: a comparison between Goldmann and rebound tonometry
Intraocular pressure measurement in mice: a comparison between Goldmann and rebound tonometry
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DOI：
10.1038/sj.eye.6702576
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发表时间：
2007-09-01
期刊：
影响因子：
3.9
通讯作者：
Kwon, Y. H.
中科院分区：
文献类型：
--
作者：
Kim, C. Y.;Kuehn, M. H.;Kwon, Y. H.
目的建立小鼠青光眼模型，需要准确测量该物种眼内压（IOP）的方法。本研究的目的是比较改良Goldmann和反弹tonometers.Methods之间的小鼠眼压测量的准确性进行了测量，无论是与改良Goldmann或反弹眼压计，同时测量眼压使用侵入性眼压计在眼球摘除和在体内。眼压水平以流体静力学控制。评价有创测压法和无创方法测定的IOP之间的一致性和相关性。此外，眼压测定两种非侵入性的方法在一个队列的小鼠与激光诱导的高眼压症（OHT），和协议和两个眼压测量方法之间的相关性进行了evaluated.Results测量眼压由任何一个非侵入性眼压计相关性以及与那些同时记录的侵入性测压（r = 0.98反弹和r = 0.94戈德曼）。在OHT小鼠中，反跳和改良Goldmann眼压计之间的IOP相关性为中度（r = 0.71）;改良Goldmann眼压计测量的IOP始终高于反跳约5 mmHg。然而，在IOP的相对百分比增加是相似的两种methods.Conclusion非侵入性的方法，在小鼠的IOP测量是合适的，以检测IOP的变化，虽然反弹眼压测量相关更好地与侵入性测压读数。结果表明，更可靠的方法，相关的研究结果，使用不同的眼压计。
Purpose The development of mouse models of glaucoma requires methods to accurately measure the intraocular pressure (IOP) in this species. The aim of this study was to compare the accuracy of IOP measurements in mice between modified Goldmann and rebound tonometers.Methods IOP was measured either with a modified Goldmann or a rebound tonometer while simultaneously measuring the IOP using invasive manometry in enucleated eyes and in vivo. The level of IOP was controlled hydrostatically. The agreement and correlation between the IOP determined by invasive manometry and by either noninvasive method was evaluated. In addition, the IOP was determined by both noninvasive methods in a cohort of mice with laser-induced ocular hypertension (OHT), and the agreement and correlation between the two tonometry methods were evaluated.Results Measured IOP by either noninvasive tonometer correlated well with those recorded simultaneously by invasive manometry (r = 0.98 for rebound and r = 0.94 for Goldmann). In mice with OHT, the IOP correlation between rebound and modified Goldmann was moderate (r = 0.71); the IOP measured by modified Goldmann tonometry was consistently higher than that by rebound by approximately 5mmHg. However, the relative per cent increases in IOP were similar between the two methods.Conclusion Both noninvasive methods of IOP measurements in mice are suitable to detect changes in IOP although rebound tonometry correlated better with the invasive manometry readings. The results suggest that more reliable means of correlating findings from studies using different tonometers.