Breast Cancer Metabolomics Using NMR
Breast Cancer Metabolomics Using NMR
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DOI：
10.1007/978-1-4939-9690-2_12
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Jagannathan, Naranamangalam R.
中科院分区：
文献类型：
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作者：
Sharma, Uma;Jagannathan, Naranamangalam R.
利用核磁共振（NMR）和体内磁共振波谱（MRS）等基于分析磁共振（MR）的方法，在了解乳腺癌代谢方面取得了持续的进展。使用这些方法的分析增强了与乳腺癌进展、消退和发病机制相关的改变的生化途径的知识。对生物样本（如组织、细胞系、细针抽吸液）和生物液体（如血清和尿液）进行全面的代谢分析，可以识别新的生物标志物和生化途径中的异常。这些方法不仅有助于癌症的诊断、治疗监测、疾病进展和分期，而且有助于确定新的治疗靶点和设计新的治疗策略。此外，体内MRS研究已经建立了含胆碱化合物（tCho）作为恶性肿瘤的生物标志物，这有助于提高磁共振成像（MRI）的诊断特异性。最近与体内磁共振相关的技术发展，如增强磁场强度、多通道相控阵乳房线圈和tCho的绝对定量，使人们更好地了解肿瘤的异质性、代谢和发病机制。本章重点介绍了用于乳腺癌代谢组学研究的体外、离体和体内MR光谱方法的实验方面。
Continued progress is being made in understanding the breast cancer metabolism using analytical magnetic resonance (MR)-based methods like nuclear magnetic resonance (NMR) and in-vivo MR spectroscopy (MRS). Analyses using these methods have enhanced the knowledge of altered biochemical pathways associated with breast cancer progression, regression, and pathogenesis. Comprehensive metabolic profiling of biological samples like tissues, cell lines, fine needle aspirate, and biofluids such as sera and urine enables identification of new biomarkers and abnormalities in biochemical pathways. These methods are not only useful for diagnosis, therapy monitoring, disease progression, and staging of cancer but also for the identification of new therapeutic targets and designing new treatment strategies. Additionally, in-vivo MRS studies have established choline-containing compounds (tCho) as biomarkers of malignancy, which is useful for enhancing the diagnostic specificity of magnetic resonance imaging (MRI). Recent technological developments related to in-vivo MRS such as increased magnetic field strength, multichannel phased array breast coils, and absolute quantification of tCho have provided a better understanding of the tumor heterogeneity, metabolism, and pathogenesis. This chapter focuses on providing the experimental aspects of in-vitro, ex-vivo, and in-vivo MR spectroscopy methods used for metabolomics studies of breast cancer.