MONOCLONAL-ANTIBODIES SPECIFIC FOR SIMIAN VIRUS-40 TUMOR-ANTIGENS
MONOCLONAL-ANTIBODIES SPECIFIC FOR SIMIAN VIRUS-40 TUMOR-ANTIGENS
复制标题
DOI：
10.1128/jvi.39.3.861-869.1981
复制
发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
WILLIAMSON, NM
中科院分区：
文献类型：
--
作者：
HARLOW, E;CRAWFORD, LV;WILLIAMSON, NM
本研究分离并克隆了30株针对SV40肿瘤抗原分泌Ig的杂交瘤。其中28个产生与SV40大T结合的抗体，2个产生与宿主53,000道尔顿蛋白结合的抗体。大T抗原至少有1个与小T抗原共有的决定因子，至少有6个独特的决定因子。来自L系列杂交瘤的一些单克隆抗体识别存在于sv40转化小鼠细胞的大T抗原亚群上的决定因子。这些单克隆抗体可用于SV40肿瘤抗原结构和功能的研究。
Thirty hybridomas that secrete Ig against the SV40 tumor antigens were isolated and cloned. Of these, 28 produced antibodies which bound to SV40 large T and 2 produced antibodies which bound to the host 53,000-dalton protein. Large T antigen had at least 1 determinant that it shared with small antigen and had a minimum of 6 unique determinants. Several of the monoclonal antibodies from the L series hybridomas recognized determinants that were present on a subset of the large T antigen from SV40-transformed mouse cells. These monoclonal antibodies should be useful in studies of the structure and function of the SV40 tumor antigens.