PROBIOTICS TREATMENT IMPROVES DIABETES-INDUCED IMPAIRMENT OF SYNAPTIC ACTIVITY AND COGNITIVE FUNCTION: BEHAVIORAL AND ELECTROPHYSIOLOGICAL PROOFS FOR MICROBIOME-GUT-BRAIN AXIS
PROBIOTICS TREATMENT IMPROVES DIABETES-INDUCED IMPAIRMENT OF SYNAPTIC ACTIVITY AND COGNITIVE FUNCTION: BEHAVIORAL AND ELECTROPHYSIOLOGICAL PROOFS FOR MICROBIOME-GUT-BRAIN AXIS
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DOI：
10.1016/j.neuroscience.2013.02.055
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发表时间：
2013-06-14
期刊：
影响因子：
3.3
通讯作者：
Salami, M.
中科院分区：
文献类型：
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作者：
Davari, S.;Talaei, S. A.;Salami, M.
糖尿病引起的代谢紊乱是许多系统活动的基础，包括大脑的一些高级功能，如学习和记忆。大量证据支持益生菌对包括神经系统在内的许多系统功能的影响。在这里，我们报告了益生菌治疗对学习和记忆障碍的行为和电生理方面的影响。腹腔注射链脲佐菌素制备糖尿病大鼠模型。对照组和糖尿病组大鼠分别用正常方案（对照组大鼠接受正常方案（CO）和糖尿病组大鼠接受正常方案（DC））或正常方案加益生菌补充剂（对照组大鼠接受益生菌补充剂（CP）和糖尿病组大鼠接受益生菌补充剂（DP））喂养2个月。首先将动物引入Morris水迷宫中的空间学习任务。然后，在电生理实验中，刺激Schaffer侧支，在海马CA 1区记录到基础和增强的兴奋性突触后电位（EPSPs）。最后测定血清葡萄糖、胰岛素、超氧化物歧化酶和8-羟基-2 '-脱氧鸟苷（8-OHdG）水平。我们发现，益生菌管理大大改善了糖尿病动物受损的空间记忆。补充益生菌可恢复糖尿病大鼠基础突触传递功能，并进一步恢复海马长时程增强（LTP）。益生菌的应用增强了超氧化物歧化酶的活性，提高了血清胰岛素水平，但降低了血清葡萄糖水平和8-OHdG因子。从目前的结果，我们得出结论，益生菌有效地扭转恶化的大脑功能的认知性能和他们提出的突触机制在糖尿病的水平。这些考虑意味着微生物组-肠-脑轴在大脑活动的行为和电生理方面的最佳功能的必要性。(C)2013年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Diabetes mellitus-induced metabolic disturbances underlie the action of many systems including some higher functions of the brain such as learning and memory. Plenty of evidence supports the effects of probiotics on the function of many systems including the nervous system. Here we report the effect of probiotics treatment on the behavioral and electrophysiological aspects of learning and memory disorders. Diabetic rats were made through intraperitoneal injection of streptozocin. The control and diabetic rats were fed with either normal regimen (control rats recieving normal regimen (CO) and diabetics rats receiving normal regimen (DC), respectively) or normal regimen plus probiotic supplementation for 2 months (control rats receiving probiotic supplementation (CP) and diabetics rats recieving probiotic supplementation (DP), respectively). The animals were first introduced to spatial learning task in the Morris water maze. Then, in electrophysiological experiments, stimulating the Schaffer collaterals the basic and potentiated excitatory postsynaptic potential (EPSPs) were recorded in the CA1 area of the hippocampus. Finally, the serum levels of glucose, insulin, superoxide dismutase and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were measured. We found that probiotics administration considerably improved the impaired spatial memory in the diabetic animals. The probiotics supplementation in the diabetic rats recovered the declined basic synaptic transmission and further restored the hippocampal long-term potentiation (LTP). While the probiotics administration enhanced the activation of superoxide dismutase and increased the insulin level of serum it decreased both the glucose level of serum and the 8-OHdG factor. From the present results we concluded that probiotics efficiently reverse deteriorated brain functions in the levels of cognitive performances and their proposed synaptic mechanisms in diabetes mellitus. These considerations imply on the necessity of an optimal function of the microbiome-gut-brain axis in the behavioral as well as electrophysiological aspects of brain action. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.