Simultaneous Nitrogen Removal and Plant Growth Promotion Using Salt-tolerant Denitrifying Bacteria in Agricultural Wastewater.
Simultaneous Nitrogen Removal and Plant Growth Promotion Using Salt-tolerant Denitrifying Bacteria in Agricultural Wastewater.
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DOI：
10.1264/jsme2.me22025
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发表时间：
2022
影响因子：
2.2
通讯作者：
中科院分区：
文献类型：
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地表沃茨中过量的硝酸盐（NO3-）和亚硝酸盐（NO2-）会对人类和环境健康产生不利影响。为了减少过量施用氮肥造成的水体污染，人们已经分离出了具有脱氮能力的细菌。为获得具有耐盐性和脱硝能力的植物根际促生菌，从植物根际土壤中分离得到一株促生菌，以番茄为试材，研究其促生效果，并在不同盐度、初始pH、碳源和农业废水条件下测定其脱硝能力。结果表明，在所检测的7个菌株中，有5个菌株显著增加了番茄植株的干重。施氏假单胞菌（Pseudomonasstutzeri）NRCB 010和Velezensis芽孢杆菌（Bacillusvelezensis）NRCB 026具有较好的促生效果、耐盐性和去除NO3--N能力。在pH为7.0时，NRCB 010和NRCB 026对NO3--N的去除率最高，分别为90.6%和92.0%。以葡萄糖或甘油为唯一碳源时，NO3--N去除率较高。NRCB 010和NRCB 026对农田污水中总氮（TN）的去除率分别为90.6%和66.7%，对养殖水体中总氮（TN）的去除率分别为79.9%和81.6%。这些结果证明了NRCB 010和NRCB 026在开发新型生物肥料及其用于减少水中氮污染方面的潜力。
Excess nitrate (NO3–) and nitrite (NO2–) in surface waters adversely affect human and environmental health. Bacteria with the ability to remove nitrogen (N) have been isolated to reduce water pollution caused by the excessive use of N fertilizer. To obtain plant growth-promoting rhizobacteria (PGPR) with salt tolerance and NO3–-N removal abilities, bacterial strains were isolated from plant rhizosphere soils, their plant growth-promoting effects were evaluated using tomato in plate assays, and their NO3–-N removal abilities were tested under different salinity, initial pH, carbon source, and agriculture wastewater conditions. The results obtained showed that among the seven strains examined, five significantly increased the dry weight of tomato plants. Two strains, Pseudomonas stutzeri NRCB010 and Bacillus velezensis NRCB026, showed good plant growth-promoting effects, salinity resistance, and NO3–-N removal abilities. The maximum NO3–-N removal rates from denitrifying medium were recorded by NRCB010 (90.6%) and NRCB026 (92.0%) at pH 7.0. Higher NO3–-N removal rates were achieved using glucose or glycerin as the sole carbon source. The total N (TN) removal rates of NRCB010 and NRCB026 were 90.6 and 66.7% in farmland effluents, respectively, and 79.9 and 81.6% in aquaculture water, respectively. These results demonstrate the potential of NRCB010 and NRCB026 in the development of novel biofertilizers and their use in reducing N pollution in water.