Pathogen invasion indirectly changes the composition of soil microbiome via shifts in root exudation profile
Pathogen invasion indirectly changes the composition of soil microbiome via shifts in root exudation profile
复制标题
病原体入侵通过根系分泌物分布的变化间接改变土壤微生物组的组成
DOI：
10.1007/s00374-016-1136-2
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发表时间：
2016-10-01
影响因子：
6.5
通讯作者：
Jousset, Alexandre
中科院分区：
文献类型：
--
作者：
Gu, Yian;Wei, Zhong;Jousset, Alexandre
植物根系分泌物调节植物与微生物的相互作用，在抑制病原菌方面可能发挥重要作用。例如，根分泌物可以直接抑制病原体或改变微生物组组成。在这里，我们测试了植物在病原体存在下是否会调节其根系分泌物，以及这些变化是否会改变根际微生物组的组成。我们从健康和青枯菌感染的番茄植物的分泌物添加到未种植的土壤中，并跟踪细菌群落组成的变化。病原菌的存在改变了酚类物质的分泌，增加了咖啡酸的释放。用受感染植物的分泌物对土壤进行改良，导致了不同的和多样性较低的土壤微生物群落的发展。至关重要的是，我们可以通过向土壤中添加纯咖啡酸来重现微生物组组成的类似变化。咖啡酸进一步抑制R.茄科植物离体生长我们的结论是，病原菌引起的根系分泌物的变化可能有助于控制病原菌直接抑制和间接转移根际微生物组的组成。
Plant-derived root exudates modulate plant-microbe interactions and may play an important role in pathogen suppression. Root exudates may, for instance, directly inhibit pathogens or alter microbiome composition. Here, we tested if plants modulate their root exudation in the presence of a pathogen and if these shifts alter the rhizosphere microbiome composition. We added exudates from healthy andRalstonia solanacearum-infected tomato plants to an unplanted soil and followed changes in bacterial community composition. The presence of pathogen changed the exudation of phenolic compounds and increased the release of caffeic acid. The amendment of soils with exudates from the infected plants led to a development of distinct and less diverse soil microbiome communities. Crucially, we could reproduce similar shift in microbiome composition by adding pure caffeic acid into the soil. Caffeic acid further suppressedR. solanacearumgrowthin vitro. We conclude that pathogen-induced changes in root exudation profile may serve to control pathogen both by direct inhibition and by indirectly shifting the composition of rhizosphere microbiome.