Reactivation of euglenoid movement and flagellar beating in detergent-extracted cells of Astasia longa: different mechanisms of force generation are involved.
Reactivation of euglenoid movement and flagellar beating in detergent-extracted cells of Astasia longa: different mechanisms of force generation are involved.
复制标题
去垢剂提取的阿斯塔西亚长加细胞中类眼虫运动和鞭毛跳动的重新激活：涉及不同的力产生机制。
作者：
T. Suzaki;R. Williamson
获得了用去垢剂提取的眼虫鞭毛虫Astasia longa 的细胞模型，该模型在添加钙时四舍五入。用 4% Triton X-100 和 Nonidet P-40 处理去除鞭毛膜、细胞体内的所有膜结构以及细胞表面凹槽区域的质膜。当游离钙的浓度升高到大于或等于10(-7)M时，诱导最大舍入，并且ATP强烈增强这种反应。对钒酸盐的离子需求和敏感性与重新激活鞭毛运动的离子需求和敏感性不同。结果表明，力产生的机制不同于鞭毛的基于动力蛋白的系统，并且细胞质游离Ca2+浓度的升高可能导致体内眼虫运动。结合其他原生动物运动系统讨论了眼虫运动的机制。
Detergent-extracted cell models of the euglenoid flagellate, Astasia longa, were obtained that rounded-up on addition of calcium. Treatment with 4% Triton X-100 and Nonidet P-40 removed the flagellar membrane, all membranous structures inside the cell body and the plasma membrane at groove regions of the cell surface. Maximum rounding-up was induced when the concentration of free calcium was raised to greater than or equal to 10(-7) M, and ATP strongly enhanced this response. The ionic requirements and sensitivity to vanadate were different from those for the reactivation of flagellar movement. The results suggest that the mechanism of force generation is different from the dynein-based system of the flagellum and that a rise in cytoplasmic free Ca2+ concentration might cause euglenoid movement in vivo. The mechanism of euglenoid movement is discussed in relation to other protozoan motile systems.