The Cotton Lignin Biosynthetic Gene Gh4CL30 Regulates Lignification and Phenolic Content and Contributes to Verticillium Wilt Resistance
The Cotton Lignin Biosynthetic Gene Gh4CL30 Regulates Lignification and Phenolic Content and Contributes to Verticillium Wilt Resistance
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DOI：
10.1094/mpmi-03-20-0071-r
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发表时间：
2021-03-01
影响因子：
3.5
通讯作者：
Sun, Jie
中科院分区：
文献类型：
--
作者：
Xiong, Xian-Peng;Sun, Shi-Chao;Sun, Jie
黄萎病是一种维管束病害，对世界范围内的棉花生产造成巨大的危害。然而，我们对棉花抗病或感病机制的了解非常有限。本研究比较了棉花品种石大路抗1号(抗黄萎病，HR)和军棉1号(抗黄萎病，HS)侵染棉花(V.dahliae，HS)前后的防御转录本，鉴定了该网络中与抗病相关的HUB基因，并对参与木质素和酚类物质生物合成的HUB基因进行了功能分析。我们鉴定了6,831个HR和HS基础转录本之间的差异表达基因，其中3,685个和3,239个在感染大丽弧菌后的不同时间点分别在HR和HS中被诱导。KEGG途径分析表明，DEGS富含木质素生物合成相关基因。根据加权基因共表达网络分析6831个基因及其在大丽弧菌感染后不同时间点的表达谱，总共鉴定出23个HUB基因。与木质素生物合成途径相关的中枢基因Gh4CL30被病毒诱导的基因沉默后，黄酮类、木质素和S单体的含量降低，G单体、G/S木质素单体、咖啡酸和阿魏酸的含量增加，从而增强了棉花对大丽叶病毒的抗性。这些结果表明，Gh4CL30是调节木质素生物合成途径不同分支产量的关键基因，为棉花对大丽叶枯病的抗性研究提供了新的思路。
Verticillium wilt is a vascular disease causing tremendous damage to cotton production worldwide. However, our knowledge of the mechanisms of cotton resistance or susceptibility to this disease is very limited. In this study, we compared the defense transcriptomes of cotton ( Gossypium hirsutum) cultivars Shidalukang 1 (Verticillium dahliae resistant, HR) and Junmian 1 (V. dahliae susceptible, HS) before and after V. dahliae infection, identified hub genes of the network associated with responses to V. dahliae infection, and functionally characterized one of the hub genes involved in biosynthesis of lignin and phenolics. We identified 6,831 differentially expressed genes (DEGs) between the basal transcriptomes of HR and HS; 3,685 and 3,239 of these DEGs were induced in HR and HS, respectively, at different time points after V. dahliae infection. KEGG pathway analysis indicated that DEGs were enriched for genes involved in lignin biosynthesis. In all, 23 hub genes were identified based on a weighted gene coexpression network analysis of the 6,831 DEGs and their expression profiles at different time points after V. dahliae infection. Knockdown of Gh4CL30, one of the hub genes related to the lignin biosynthesis pathway, by virus-induced gene silencing, led to a decreased content of flavonoids, lignin, and S monomer but an increased content of G monomer, G/S lignin monomer, caffeic acid, and ferulic acid, and enhanced cotton resistance to V. dahliae. These results suggest that Gh4CL30 is a key gene modulating the outputs of different branches of the lignin biosynthesis pathway, and provide new insights into cotton resistance to V. dahliae.