Cationic porphyrins as probes of DNA structure.
Cationic porphyrins as probes of DNA structure.
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阳离子卟啉作为 DNA 结构的探针。
DOI：
10.1093/nar/14.22.9133
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发表时间：
1986
影响因子：
14.9
通讯作者：
Dabrowiak,JC
中科院分区：
文献类型：
--
作者：
Bromley,SD;Ward,BW;Dabrowiak,JC
一组阳离子锰卟啉复合物的DNA结合特异性已经通过DNA酶I足迹方法和观察在超氧化物钾存在下卟啉诱导的DNA链断裂的位点进行了研究。这些化合物在DNA中富含AT的区域的小凹槽中结合，在总电荷、大环上的外围分布和配体形状上都有系统的变化。虽然总电荷和电荷排列的变化对特异性没有显著影响，但阻止配体与小凹槽紧密接触的形状变化会使原始AT特异性松弛，导致化合物在AT和GC位点都发生裂解。观察到的结合序列特异性的变化是根据与化合物和DNA相关的静电和空间因素来解释的。
The DNA binding specificity of a group of cationic manganese porphyrin complexes has been examined using DNase I footprinting methodology and by observing the sites of porphyrin-induced DNA strand scission in the presence of potassium superoxide. The compounds, which possess systematic changes in total charge, its distribution on the periphery on the macrocycle and ligand shape, bind in the minor groove of AT rich regions of DNA. While changes in total charge and charge arrangement do not significantly influence specificity, a shape change which blocks close ligand contact with the minor groove relaxes the original AT specificity causing the compound to cleave at both AT and GC sites. The observed changes in binding sequence specificity were interpreted in terms of electrostatic and steric factors associated with both the compounds and DNA.