HA-RAS ONCOGENES ARE ACTIVATED BY SOMATIC ALTERATIONS IN HUMAN URINARY-TRACT TUMORS
HA-RAS ONCOGENES ARE ACTIVATED BY SOMATIC ALTERATIONS IN HUMAN URINARY-TRACT TUMORS
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DOI：
10.1038/309464a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
AARONSON, SA
中科院分区：
文献类型：
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作者：
FUJITA, J;YOSHIDA, O;AARONSON, SA
以NIH3T3细胞为受体的DNA介导的基因转移(转染法)研究已经证明在各种人类肿瘤中存在转化基因(癌基因)(综述见文献1，2)。到目前为止，通过DNA转染检测到的癌基因中有很大一部分与Harvey(和B ALB)小鼠肉瘤病毒(MSV)3-6的Ha-ras癌基因、Kirsten MSV3、7、8的癌基因Ki-ras以及该基因家族的第三个成员N-ras有关(参考文献8-12)。许多不同器官的单个肿瘤与基因家族成员的激活有关。现在，我们首次对手术后立即处理的人类尿路肿瘤以及来自相同患者的正常组织进行系统调查，以检测这些基因的存在。我们证明了在大约10%的随机选择的尿路肿瘤中，癌基因Ha-rasas被激活，并且有直接证据表明，癌基因的激活是在肿瘤细胞群体中被选择的躯体事件的结果。
DNA-mediated gene transfer (transfection) studies using NIH 3T3 cells as recipients have demonstrated the presence of transforming genes (oncogenes) in diverse human tumours (for reviews see refs 1, 2). A large proportion of oncogenes so far detected by DNA transfection are related to the Ha-ras oncgene of Harvey (and B ALB) murine sarcoma viruses (MSV)3–6, Ki-ras, the oncogene of Kirsten MSV3,7,8, and a third member of therasgene family, N-ras(refs 8–12). Individual tumours of many different organs have been associated with the activation of members of therasgene family. We now present the first systematic survey of human urinary tract tumours processed immediately after surgery, as well as normal tissues from the same patients, to detect the presence of such genes. We demonstrate activation of Ha-rasas an oncogene in around 10% of randomly selected urinary tract tumours as well as direct evidence that oncogene activation is the result of a somatic event which is selected for within the tumour cell population.