Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation
Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation
复制标题
束缚应激改变免疫参数并在胚胎植入过程中诱导小鼠子宫氧化应激
DOI：
10.3109/10253890.2014.966263
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发表时间：
2014-12-01
影响因子：
2.3
通讯作者：
Chen, Yaoxing
中科院分区：
文献类型：
--
作者：
Liu, Guanhui;Dong, Yulan;Chen, Yaoxing
摘要应激对胚胎着床的影响尚不清楚。此前的研究主要集中在妊娠后期和压力对免疫功能的长期影响。本研究的目的是探讨束缚应激对着床期子宫免疫指标和氧化状态的影响。在这项研究中，怀孕的CD 1小鼠进行约束应激（4小时/天）的胚胎第1天（E1）和E3，E5和E7处死。检查母体血浆皮质酮（CORT）分泌和子宫着床部位。收集子宫（不包括胚胎）匀浆和子宫淋巴细胞，以检查氧化应激状态和相关的免疫参数。结果表明，束缚应激增加母体血浆CORT分泌，并减少了E5和E7的着床位点数分别为15.3%和26.1%。此外，束缚应激使子宫内膜中的子宫自然杀伤（uNK）细胞密度降低22.1-47.9%，使子宫肌层中的肥大细胞密度增加55.6- 76.9%。束缚应激可显著降低CD 3 + CD 4 + T/CD 3 + CD 8 + T细胞比值（降低26.2-28.9%），抑制子宫淋巴细胞增殖和细胞因子分泌。此外，束缚应激威胁到细胞内氧化剂和抗氧化剂之间的平衡，导致谷胱甘肽过氧化物酶（GSH-PX）降低（32.2%和45.7%），超氧化物歧化酶（SOD）总抗氧化能力（T-AOC）活性（18.4%和18.2%）和丙二醛（MDA）含量（34.4%和43.0%）增加。总之，这些研究结果表明，束缚应激导致异常植入，并对小鼠中与氧化应激相关的免疫参数产生负面影响。
Abstract The influence of stress on embryo implantation is not well understood. Prior studies have focused on later gestational stages and the long-term impact of stress on immune function. The objective of this study is to investigate the effects of restraint stress on the immune parameters and the oxidative states of the uterus during implantation. In this study, pregnant CD1 mice were subjected to restraint stress (4 h/d) on embryonic day 1 (E1) and sacrificed on E3, E5, and E7. Maternal plasma corticosterone (CORT) secretion and implantation sites in the uterus were examined. The uterine (excluding embryos) homogenate and uterine lymphocytes were collected to examine oxidative stress states and associated immune parameters. The results demonstrated that restraint stress increased maternal plasma CORT secretion and reduced the number of implantation sites by 15.3% on E5 and by 26.1% on E7. Moreover, restraint stress decreased the density of uterine natural killer (uNK) cells in the endometrium by 22.1–47.9% and increased the density of mast cells in the myometrium by 55.6–76.9%. Restraint stress remarkably decreased the CD3+CD4+ T/CD3+CD8+ T cell ratio (by 26.2–28.9%) and attenuated uterine lymphocyte proliferation and secretion of cytokines. In addition, restraint stress threatened the intracellular equilibrium between oxidants and antioxidants, resulting in decreased glutathione peroxidase (GSH-PX) (32.2% and 45.7%), superoxide dismutase (SOD) (15.5% and 26.1%), and total antioxidant capacity (T-AOC) (18.4% and 18.2%) activities and increased malondialdehyde (MDA) (34.4% and 43.0%) contents on E5 and E7. In conclusion, these findings demonstrate that restraint stress causes abnormal implantation and negatively impacts immune parameters in association with oxidative stress in mice.