Alteration of lipid organization following fertilization of sea urchin eggs.
Alteration of lipid organization following fertilization of sea urchin eggs.
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海胆卵受精后脂质组织的改变。
DOI：
10.1016/0167-4889(84)90009-0
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Williamson,P
中科院分区：
文献类型：
--
作者：
Freidus,DJ;Schlegel,RA;Williamson,P
荧光探针mercury 540被用来检查海胆卵受精后质膜的外部小叶中的脂质的组织。这些脂质在未受精卵中紧密堆积，如它们不能结合染料所证明的，而在受精卵和至少到原肠胚阶段的胚胎细胞中，膜变得更松散组织，并且染色有明亮的环形荧光。用氨诱导晚期受精事件未能诱导染色行为的这种变化。由于孤雌激活的卵子染色，因此不需要精子成分来诱导这种改变。然而，普鲁卡因，它专门抑制皮质颗粒融合的早期事件，鸡蛋的治疗是有效的抑制染色。这些结果表明，受精后的皮质颗粒融合导致海胆卵质膜脂质组织的变化。
The fluorescent probe merocyanine 540 was used to examine the organization of the lipids in the external leaflet of the plasma membrane after fertilization of sea urchin eggs. These lipids in unfertilized eggs are closely packed, as evidenced by their inability to bind the dye, whereas in fertilized eggs and cells of embryos up to at least the gastrula stage, the membrane becomes more loosely organized, and stains with bright ring fluorescence. Induction of late fertilization events with ammonia failed to induce this change in staining behavior. Sperm components are not required to induce this alteration since parthenogenetically activated eggs stained. However, treatment of eggs with procaine, which specifically inhibits the early event of cortical granule fusion, was effective in suppressing staining. These results indicate that cortical granule fusion after fertilization results in a change in the organization of the lipids of the plasma membrane of sea urchin eggs.