Ribozymes, the first 20 years
Ribozymes, the first 20 years
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DOI：
10.1042/bst0301162
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发表时间：
2002-11-01
影响因子：
3.9
通讯作者：
Cech, TR
中科院分区：
文献类型：
--
作者：
Cech, TR
1982年，我们报道了第一个催化RNA或核酶：四膜虫前RNA的自剪接内含子。很快就发现了天然核酶的其他例子，该领域的研究集中在它们的酶机制和二级和三级结构上。通过体外筛选鉴定的核酶扩展了RNA催化的功能。目前和未来的两个方向是通过x射线晶体学确定大型核酶的原子分辨率结构，以及核酶与蛋白质促进物的结构和机制分析。
In 1982 we reported the first catalytic RNA or ribozyme: the self-splicing intron of the Tetrahymena pre-rRNA. Additional examples of natural ribozymes were soon found, and research in the field focused on their enzymic mechanism and secondary and tertiary structure. Ribozymes identified through in vitro selection extended the repertoire of RNA catalysis. Two directions of current and future interest are the determination of atomic-resolution structures of large ribozymes by X-ray crystallography and the structural and mechanistic analysis of complexes of ribozymes with protein facilitators of their activity.