A high-fructose diet induces epithelial barrier dysfunction and exacerbates the severity of dextran sulfate sodium-induced colitis
A high-fructose diet induces epithelial barrier dysfunction and exacerbates the severity of dextran sulfate sodium-induced colitis
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DOI：
10.3892/ijmm.2018.4040
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发表时间：
2019-03-01
影响因子：
5.4
通讯作者：
Ido, Akio
中科院分区：
文献类型：
--
作者：
Kawabata, Katsuto;Kanmura, Shuji;Ido, Akio
过量果糖摄入是炎症性肠病患者出现肠道症状的危险因素，但此前尚未评估其对肠道的影响。本研究调查了高果糖饮食（HFD）对实验性结肠炎小鼠肠道屏障功能的影响。为 C57/BL6 小鼠提供 HFD 或对照饮食以及普通饮用水或含有 1% 葡聚糖硫酸钠 (DSS) 的水，为期 2 周。比较各组间疾病活动指数（DAI）、病理评分、炎性细胞因子表达量，并分析结肠中粪便细菌比例。与非 DSS 处理和对照饮食小鼠相比，DSS/HFD 处理的小鼠体重和结肠长度显着降低，DAI 和病理评分显着增加。关于炎症细胞因子的表达，DSS/HFD处理的小鼠中白细胞介素(IL)-6、IL-1和肿瘤坏死因子-的水平显着升高，而紧密连接蛋白occludin的表达显着降低。 HFD 小鼠的细菌总数增加。总而言之，这些结果表明，HFD 会导致肠道屏障功能恶化，并增加对 DSS 诱导的结肠炎的易感性。
Excessive fructose intake is a risk factor for gut symptoms in patients with inflammatory bowel disease, however, its effect on the intestinal tract has not been evaluated previously. The present study investigated the impact of a high-fructose diet (HFD) on intestinal barrier function in mice with experimental colitis. C57/BL6 mice were provided with either a HFD or control diet and either plain drinking water or water containing 1% dextran sulfate sodium (DSS) for 2 weeks. The disease activity index (DAI), pathological scores and expression of inflammatory cytokines were compared among the groups, and the proportions of fecal bacteria in the colon were analyzed. The body weight and colon length were significantly decreased, and the DAI and pathological scores were significantly increased in the DSS/HFD-treated mice compared with the non-DSS-treated and control diet mice. Regarding the expression of inflammatory cytokines, the levels of interleukin (IL)-6, IL-1 and tumor necrosis factor- were significantly increased, and the expression of the tight junction protein occludin was significantly decreased in the DSS/HFD-treated mice. The total bacterial count was increased in the HFD mice. Taken together, these results indicate that an HFD resulted in the deterioration of intestinal barrier function and increased susceptibility to DSS-induced colitis.