IMMUNIZATION WITH WHOLE INACTIVATED VACCINE PROTECTS FROM INFECTION BY SIV GROWN IN HUMAN BUT NOT MACAQUE CELLS
IMMUNIZATION WITH WHOLE INACTIVATED VACCINE PROTECTS FROM INFECTION BY SIV GROWN IN HUMAN BUT NOT MACAQUE CELLS
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DOI：
10.1111/j.1600-0684.1994.tb00105.x
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发表时间：
1994-02-01
影响因子：
0.7
通讯作者：
HIRSCH, VM
中科院分区：
文献类型：
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作者：
GOLDSTEIN, S;ELKINS, WR;HIRSCH, VM
通过在人CEM x 174细胞或猕猴外周血单核细胞中传代SIV（sm）的猕猴分离株（SIV（sm/E660））来产生猕猴SIV（sm）原液。用由用SIV（smH 4）克隆转染CEM x 174细胞产生的病毒组成的全灭活SIV疫苗免疫猕猴，并用任一无细胞储备液攻击猕猴。只有用人类细胞中产生的病毒攻击的疫苗接种者才能免受感染。这证实了全灭活疫苗介导的保护的种属特异性。
Homologous SIV(sm) stocks were generated by passage of a macaque isolate of SIV(sm) (SIV(sm/E660)) in human CEM x 174 cells or macaque peripheral blood mononuclear cells. Macaques were immunized with whole inactivated SIV vaccine consisting of virus generated by transfection of CEM x 174 cells with the SIV(smH4) clone and were challenged with either cell-free stock. Only vaccinees challenged with virus generated in human cells were protected from infection. This confirms the species-specificity of whole inactivated vaccine-mediated protection.