Mechanisms of vertebrate neural plate internalization
Mechanisms of vertebrate neural plate internalization
复制标题
DOI：
10.1387/ijdb.200122ca
复制
发表时间：
2021-01-01
影响因子：
0.7
通讯作者：
Carrasco, Daniela
中科院分区：
文献类型：
--
作者：
Araya, Claudio;Carrasco, Daniela
多细胞组织的内化是动物发育和器官形成过程中的关键形态发生过程。这方面的一个很好的例子是脊椎动物中枢神经系统形成的初始阶段，其中称为神经板的瞬时胚胎结构能够在背中线内进行集体细胞重排。尽管神经板中线内化的缺陷可能导致一系列严重的临床状况，如脊柱裂和无脑畸形，但这个过程的生化和生物力学细节仍然只有部分特征。本文就脊椎动物神经管形成过程中中线细胞和组织内化的主要细胞和分子机制进行综述。我们讨论了集体细胞机制的贡献，包括会聚和伸展，以及促进中线神经板成形的顶端收缩。此外，我们总结了最近的研究，揭示了如何相互作用的信号通路和细胞生物力学调节神经板内化。此外，我们讨论了如何粘附依赖性细胞接触似乎是一个关键组成部分，中线细胞收敛和表面细胞收缩通过细胞-细胞机械耦合。我们设想，更详细的高分辨率的定量数据，在细胞和组织水平将需要适当的建模脊椎动物神经板内化的机制，希望防止人类神经管缺陷。
The internalization of multi-cellular tissues is a key morphogenetic process during animal development and organ formation. A good example of this is the initial stages of vertebrate central nervous system formation whereby a transient embryonic structure called the neural plate is able to undergo collective cell rearrangements within the dorsal midline. Despite the fact that defects in neural plate midline internalization may result in a series of severe clinical conditions, such as spina bifida and anencephaly, the biochemical and biomechanical details of this process remain only partially characterized. Here we review the main cellular and molecular mechanisms underlying midline cell and tissue internalization during vertebrate neural tube formation. We discuss the contribution of collective cell mechanisms including convergence and extension, as well as apical constriction facilitating midline neural plate shaping. Furthermore, we summarize recent studies that shed light on how the interplay of signaling pathways and cell biomechanics modulate neural plate internalization. In addition, we discuss how adhesion-dependent cell-cell contact appears to be a critical component during midline cell convergence and surface cell contraction via cell-cell mechanical coupling. We envision that more detailed high-resolution quantitative data at both cell and tissue levels will be required to properly model the mechanisms of vertebrate neural plate internalization with the hope of preventing human neural tube defects.