High Expression of Derlin-1 Is Associated with the Malignancy of Bladder Cancer in a Chinese Han Population.
High Expression of Derlin-1 Is Associated with the Malignancy of Bladder Cancer in a Chinese Han Population.
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Derlin-1的高表达与中国汉族人群膀胱癌的恶性相关。
DOI：
10.1371/journal.pone.0168351
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wang SL
中科院分区：
文献类型：
--
作者：
Wu Z;Wang C;Zhang Z;Liu W;Xu H;Wang H;Wang Y;Zhang W;Wang SL
Derlin-1在各种类型的实体瘤中过表达，在癌症进展中具有重要作用。然而，其在膀胱癌中的表达模式及其与膀胱癌临床病理特征的关系尚不清楚。本研究首先采用液相色谱串联质谱（UPLC-MS/MS）技术对3对新鲜膀胱癌组织和癌旁组织进行检测，筛选差异表达蛋白。在通过qRT-PCR和蛋白质印迹进行生物信息学分析和评估后，选择Derlin-1作为候选蛋白，然后通过免疫组织化学和蛋白质印迹在来自膀胱癌患者的样品中进行验证。结果显示，膀胱癌组织中Derlin-1的表达水平明显高于癌旁组织（P <0.05）。Derlin-1的阳性表达与肿瘤分期、组织学分级和淋巴结转移显著相关（P <0.001），而与患者年龄（P = 0.758）和性别（P = 0.831）等临床病理参数无关。此外，Derlin-1在BC细胞系（um-uc-3和T24）中高表达，干扰Derlin-1可逆转T24细胞EMT进程，抑制肿瘤的迁移和侵袭。Derlin-1阳性表达的患者总生存期较阴性表达的患者短（P <0.001）。综上所述，我们的结果表明，Derlin-1在膀胱癌中过表达，并与膀胱癌的恶性程度有关。
Derlin-1 is overexpressed in various types of solid tumors and has an important function in cancer progression. However, its expression pattern in and association with the clinicopathological characteristics of human bladder cancer remain unclear. In the present study, 3 pairs of fresh samples of bladder cancer tissue and paracancerous tissue were first detected by liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to screen for differentially expressed proteins. Following bioinformatics analysis and assessments by qRT-PCR and western blotting, Derlin-1 was selected as a candidate protein and was then validated in samples from patients with bladder cancer by immunohistochemistry and western blotting. The results showed that the bladder cancer tissues exhibited higher levels of Derlin-1 expression than the paracancerous tissues (P < 0.05). Positive expression of Derlin-1 was significantly correlated with tumor stage, histological grade, and lymph node metastasis (P < 0.001) but was not correlated with other clinicopathological parameters including patient age (P = 0.758) and gender (P = 0.831). Besides, Derlin-1 was highly expressed in BC cell lines (um-uc-3 and T24), and the interference of Derlin-1 could reverse EMT progression, inhibit the tumor migration and invasion in T24 cells. Further, patients with positive Derlin-1 expression had shorter overall survival than those with negative expression (P < 0.001). Taken together, our results demonstrated that Derlin-1 was overexpressed in bladder cancer and was associated with the malignancy of bladder cancer.
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影响因子：
64.8
作者：
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通讯作者：
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