Human in vivo fluorescence microimaging of the alveolar ducts and sacs during bronchoscopy
Human in vivo fluorescence microimaging of the alveolar ducts and sacs during bronchoscopy
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DOI：
10.1183/09031936.00083708
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发表时间：
2009-05-01
影响因子：
24.3
通讯作者：
Bourg-Heckly, G.
中科院分区：
文献类型：
--
作者：
Thiberville, L.;Salauen, M.;Bourg-Heckly, G.
本研究的目的是评估纤维共焦荧光显微镜 (FCFM) 作为支气管镜检查期间体内肺泡呼吸系统成像的工具。488 nm 激发波长 FCFM 装置用于 41 名健康受试者（包括 17 名主动吸烟者）。表面麻醉后，将 1.4 毫米微型探针引入支气管镜工作通道，并向远端推进至肺泡。对具有最小压缩效应的选定框架进行形态测量和细胞分析。从除了两个上叶的心尖和后段之外的每个肺段获得体内腺泡显微成像。从 192 个单独的腺泡中记录了与轴向和周围间质系统的弹性框架相对应的可重复模式。腺泡弹性纤维的平均+/-SD厚度为10+/-2.7μm。肺泡口直径（平均值+/-SD 278+/-53μm）呈正态分布，但右上叶和右内侧基底段显得较小。小叶微血管（中位直径90μm）均匀分布在整个肺部。在非吸烟者中检测不到肺泡巨噬细胞，而在吸烟者中观察到特定的烟草焦油诱导的荧光，提供了肺泡壁和巨噬细胞的精细细节。研究发现，每天吸烟的数量与体内观察到的大型移动巨噬细胞的数量以及巨噬细胞肺泡炎的强度之间存在很强的相关性。纤维共聚焦荧光显微镜能够准确地探索吸烟者和非吸烟者体内的外周肺。
The aim of the present study was to assess fibred confocal fluorescence microscopy (FCFM) as a tool for imaging the alveolar respiratory system in vivo during bronchoscopy.A 488-nm excitation wavelength FCFM device was used in 41 healthy subjects including 17 active smokers. After topical anaesthesia, the 1.4-mm miniprobe was introduced into the bronchoscope working channel and advanced distally to the alveoli. Morphometric and cellular analyses were performed on selected frames harbouring a minimal compression effect.In vivo acinar microimaging was obtained from each lung segment except for the apical and posterior segments of both upper lobes. Reproducible patterns, corresponding to the elastic framework of the axial and peripheral interstitial systems, were recorded from 192 separate acini. The mean +/- SD thickness of the acinar elastic fibres was 10 +/- 2.7 mu m. Alveolar mouth diameters (mean +/- SD 278 +/- 53 mu m) were normally distributed but appeared smaller in the right upper lobe and right medial basal segment. Lobular microvessels (median diameter 90 mu m) were equally distributed throughout the lungs. Alveolar macrophages were not detectable in nonsmokers, whereas a specific tobacco-tar-induced fluorescence was observed in smoking subjects, providing fine details of the alveolar walls and macrophages. A strong correlation was found between the number of cigarettes smoked per day and the amount of large and mobile macrophages observed in vivo, as well as with the intensity of the macrophage alveolitis.Fibred confocal fluorescence microscopy enables accurate exploration of the peripheral lung in vivo in both smokers and nonsmokers.