Toxico-pathological changes and phytochemically-induced alleviation in Diabetic Rats Treated With Gongronema latifolium Leaf Extracts
Toxico-pathological changes and phytochemically-induced alleviation in Diabetic Rats Treated With Gongronema latifolium Leaf Extracts
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用贡格罗玛叶提取物治疗糖尿病大鼠的毒理学变化和植物化学诱导的缓解
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
F. Uboh
中科院分区：
文献类型：
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作者：
E. Edet;T. E. Edet;M. Akpanabiatu;E. David;I. Atangwho;G. Igile;B. Mgbeje;F. Uboh
这项工作评估了Gongronema latifolium Benth et Hook（萝藦科）叶提取物对糖尿病和非糖尿病雄性大鼠的胰腺、肾脏、心脏和肝脏组织学的影响。糖尿病大鼠的胰腺表现出β细胞密度和大小减少、网状支撑扭曲和发炎细胞浸润，但在用400mg/kg体重提取物治疗后，胰腺得到恢复，显示出明显且高度密集的β和αlpha细胞。糖尿病大鼠肾脏的肾小管出现炎症，并且曲小管之间的边界模糊。然而，灌胃提取物可使这些迄今发炎的细胞恢复和再生。在此期间，高血糖和提取物给药没有引起心脏组织的显着变化，只是提取物处理的大鼠的肌细胞出现多核和肥大。肝脏的组织学也没有观察到显着的变化。叶提取物的植物化学筛选结果显示，乙醇和水提取物中都存在生物碱、皂苷、单宁、叶草单宁、强心苷、还原糖和多酚。然而，与水提取物相比，乙醇提取物中的多酚和皂苷相对较高。提取物针对高血糖引起的组织毒性的可能的再生、保护和恢复作用可能归因于这些植物化学物质。
This work assessed the effect of Gongronema latifolium Benth et Hook (Asclepiadaceae) leaf-extract on the histology of pancreas, kidney, heart and liver tissues of diabetic and non-diabetic male rats. The pancreas of diabetic rats which showed reduction in beta-cell density and size, distorted reticular support and infiltration of inflamed cells, recover ed, showing distinct and highly populated beta- and α lpha-cells upon treatment with 400mg/kg body wt ext ract. The renal tubules of the diabetic rat kidneys indicated inflammation and obscured borders between convoluted tubules. However, gavaging with the extract caused a restoration and regeneration of th ese hitherto inflamed cells. Hyperglycaemia as well as extract administration caused no significant cha nge in the heart tissues within the period, except that the myocytes of the extract-treated rats were multinucleated and hypertrophied. No significant change was also observed in the histology of the li ver. Result of phytochemical screening of leaf- extract revealed the presence of alkaloids, saponin s, tannins, phlobatannins, cardiac glycosides, reducing sugars and polyphenols in both ethanol and aqueous extracts. However, polyphenols and saponins were relatively higher in ethanol extract compared to the aqueous. The possible regenerative, protective and restorative effect of extract agains t hyperglycaemia-induced tissue toxicity may be attributable to these phytochemicals.