Quantitative assessment of apoptosis in oral lichen planus
Quantitative assessment of apoptosis in oral lichen planus
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DOI：
10.1016/s1079-2104(99)70116-2
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发表时间：
1999-08-01
期刊：
影响因子：
--
通讯作者：
Morgan, PR
中科院分区：
文献类型：
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作者：
Bloor, BK;Malik, FK;Morgan, PR
Objective.本研究的目的是通过原位末端标记来检测口腔扁平苔藓中淋巴细胞浸润的频率，以确定该技术是否与传统的组织学分析一样敏感，并检测淋巴细胞浸润的影响。将苏木精-伊红染色切片中的凋亡数目与口腔扁平苔藓中原位末端标记鉴定的凋亡细胞核数目进行比较（n = 26）正常颊黏膜8例。MIB-1、Bcl-2和Bar的免疫组化染色使可能的调控途径得以研究。在口腔扁平苔藓中，每毫米基底层检测到约1个凋亡细胞，细胞死亡随着淋巴细胞浸润而增加。上皮细胞增殖与凋亡无相关性。Bcl-2在基底细胞表达较弱或不表达，Bar在棘细胞表达较强。在口腔扁平苔藓中检测到数量增加的淋巴结转移，特别是与淋巴细胞浸润相关，苏木精-伊红染色比原位末端标记观察到更多的淋巴结转移。
Objective. The aims of this study were to examine the frequency of apoptoses in oral lichen planus by in situ end labeling, to ascertain whether this technique is as sensitive as conventional histologic analysis, and to examine the effect of lymphocytic infiltration.Study design, Numbers of apoptoses in hematoxylin-eosin stained sections were compared with numbers of apoptotic nuclei identified by in situ end labeling in oral lichen planus (n = 26) and normal buccal epithelium (n = 8). Immunohistochemical staining with MIB-1 and for Bcl-2 and Bar enabled possible regulatory pathways to be investigated.Results. In oral lichen planus, approximately 1 apoptotic cell was detected per millimeter of basal layer, cell death increasing with lymphocytic infiltration. Epithelial cell proliferation did not correlate with apoptosis. Bcl-2 expression was weak or absent in basal cells, and Bar was localized to upper prickle cells.Conclusions. Increased numbers of apoptoses were detected in oral lichen planus, especially in association with lymphocytic infiltration, higher numbers being seen with hematoxylin-eosin staining than with in situ end labeling.