RITA Inhibits Growth of Human Hepatocellular Carcinoma Through Induction of Apoptosis
RITA Inhibits Growth of Human Hepatocellular Carcinoma Through Induction of Apoptosis
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DOI：
10.3727/096504013x13685487925059
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Liu, Chunbo
中科院分区：
文献类型：
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作者：
Wang, Haihe;Chen, Guofu;Liu, Chunbo
RBP-J 相互作用和微管蛋白相关 (RITA) 是一种新型 RBP-J 相互作用蛋白，可下调 Notch 介导的转录。目前的研究重点是RITA在人肝细胞癌（HCC）中的抗肿瘤作用，旨在探讨其分子机制。通过实时定量逆转录酶聚合酶链反应 (qRT-PCR) 分析了 30 对 HCC 和邻近的非肿瘤肝脏样本。通过将 pcDNA3.1-Flag-RITA 质粒转染到 HepG2 细胞中来诱导 RITA 过表达。 RITA 敲低是通过 siRNA 转染实现的。分别通过 qRT-PCR 和 Western blotting 定量靶基因的 mRNA 和蛋白表达。使用MTT测定和流式细胞术测量细胞增殖和凋亡。我们的结果表明，与 HCC 组织相比，邻近的非肿瘤肝脏样本表现出 RITA 表达增加 (p
RBP-J-interacting and tubulin-associated (RITA) is a novel RBP-J-interacting protein that downregulates Notch-mediated transcription. The current study focuses on the antitumor effect of RITA in human hepatocellular carcinoma (HCC) and aims to explore its molecular mechanism. Thirty paired HCC and adjacent nontumoral liver samples were analyzed by real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). RITA overexpression was induced by transfection of a pcDNA3.1-Flag-RITA plasmid into HepG2 cells. RITA knockdown was achieved by siRNA transfection. mRNA and protein expression of target genes were quantified by qRT-PCR and Western blotting, respectively. Cell proliferation and apoptosis were measured using MTT assay and flow cytometry. Our results demonstrate that adjacent nontumoral liver samples exhibited increased RITA expression compared to HCC tissues (p