Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.
Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.
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DOI：
10.1038/s41467-021-26000-9
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发表时间：
2021-10-01
影响因子：
16.6
通讯作者：
Lilley KS
中科院分区：
文献类型：
--
作者：
Mulvey CM;Breckels LM;Crook OM;Sanders DJ;Ribeiro ALR;Geladaki A;Christoforou A;Britovšek NK;Hurrell T;Deery MJ;Gatto L;Smith AM;Lilley KS
Protein localisation and translocation between intracellular compartments underlie almost all physiological processes. The hyperLOPIT proteomics platform combines mass spectrometry with state-of-the-art machine learning to map the subcellular location of thousands of proteins simultaneously. We combine global proteome analysis with hyperLOPIT in a fully Bayesian framework to elucidate spatiotemporal proteomic changes during a lipopolysaccharide (LPS)-induced inflammatory response. We report a highly dynamic proteome in terms of both protein abundance and subcellular localisation, with alterations in the interferon response, endo-lysosomal system, plasma membrane reorganisation and cell migration. Proteins not previously associated with an LPS response were found to relocalise upon stimulation, the functional consequences of which are still unclear. By quantifying proteome-wide uncertainty through Bayesian modelling, a necessary role for protein relocalisation and the importance of taking a holistic overview of the LPS-driven immune response has been revealed. The data are showcased as an interactive application freely available for the scientific community. “Protein relocalisation plays a major role in the innate immune response but remains incompletely characterised. Here, the authors combine temporal proteomics with LOPIT, a spatial proteomic workflow, in a fully Bayesian framework to elucidate spatiotemporal proteomic changes during the LPS-induced immune response in THP-1 cells.
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影响因子：
7.4
作者：
Da Riva L;Bozzi F;Mondellini P;Miccichè F;Fumagalli E;Vaghi E;Tarantino E;Huber V;Gronchi A;Tamborini E;Pierotti MA;Pilotti S;Bongarzone I
通讯作者：
Bongarzone I
影响因子：
4.3
作者：
Breckels LM;Holden SB;Wojnar D;Mulvey CM;Christoforou A;Groen A;Trotter MW;Kohlbacher O;Lilley KS;Gatto L
通讯作者：
Gatto L
影响因子：
16.6
作者：
Christoforou A;Mulvey CM;Breckels LM;Geladaki A;Hurrell T;Hayward PC;Naake T;Gatto L;Viner R;Martinez Arias A;Lilley KS
通讯作者：
Lilley KS
影响因子：
4.3
作者：
Crook OM;Geladaki A;Nightingale DJH;Vennard OL;Lilley KS;Gatto L;Kirk PDW
通讯作者：
Kirk PDW
影响因子：
4.4
作者：
Caprara, Greta;Prosperini, Elena;Natoli, Gioacchino
通讯作者：
Natoli, Gioacchino