The role of the resting zone in growth plate chondrogenesis
The role of the resting zone in growth plate chondrogenesis
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DOI：
10.1210/en.143.5.1851
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发表时间：
2002-05-01
期刊：
影响因子：
4.8
通讯作者：
Baron, J
中科院分区：
文献类型：
--
作者：
Abad, V;Meyers, JL;Baron, J
在哺乳动物中，长骨的生长发生在生长板上，生长板是一种笼状结构，包含三个主要层：静止区、增殖区和肥大区。休息区的功能还没有被很好地理解。我们在体内去除了兔尺骨远端生长板上的增殖区和肥大区，只留下了休息区。在1wk内，常有完整的增殖带和肥大带再生。下一步，我们在体内操纵生长板，将休息区软骨异位放置在增殖柱旁边。异位静止区软骨诱导邻近增殖区软骨细胞方向发生90度移位，并抑制其肥大分化。我们的研究结果表明，静止区软骨对生长板软骨内骨的形成有重要贡献：1)它含有干细胞样细胞，可以形成增殖软骨细胞的克隆；2)它产生一种生长板定向因子，一种形态原，引导增殖克隆排列成平行于骨长轴的柱状；3)它还可能产生一种形态原，抑制附近增殖区软骨细胞的终末分化，从而可能是生长板组织成不同的增殖区和肥大区的部分原因。
In mammals, growth of long bones occurs at the growth plate, a catilage structure that contains three principal layers: the resting, proliferative, and hypertrophic zones. The function of the resting zone is not well understood. We removed the proliferative and hypertrophic zones from the rabbit distal ulnar growth plate in vivo, leaving only the resting zone. Within 1 wk, a complete proliferative and hypertrophic zone often regenerated. Next, we manipulated growth plates in vivo to place resting zone cartilage ectopically alongside the proliferative columns. Ectopic resting zone cartilage induced a 90-degree shift in the orientation of nearby proliferative zone chondrocytes and seemed to inhibit their hypertrophic differentiation. Our findings suggest that resting zone cartilage makes important contributions to endochondral bone formation at the growth plate: 1) it contains stem-like cells that give rise to clones of proliferative chondrocytes; 2) it produces a growth plate-orienting factor, a morphogen, that directs the alignment of the proliferative clones into columns parallel to the long axis of the bone; and 3) it may also produce a morphogen that inhibits terminal differentiation of nearby proliferative zone chondrocytes and thus may be partially responsible for the organization of the growth plate into distinct zones of proliferation and hypertrophy.