A sensitive aptasensor for the detection of beta-amyloid oligomers based on metal-organic frameworks as electrochemical signal probes
A sensitive aptasensor for the detection of beta-amyloid oligomers based on metal-organic frameworks as electrochemical signal probes
复制标题
基于金属有机框架作为电化学信号探针的用于检测β-淀粉样蛋白寡聚体的灵敏适体传感器
作者：
Zhou Y. L.;Li C. M.;Li X. Q.;Zhu X.;Ye B. X.;Xu M. T.
在这项研究中，我们开发了一种新型且简便的电化学适体传感器，用于基于金属有机框架（MOF）作为信号探针的β-淀粉样蛋白（Aβ）低聚物检测。通过使用适配体系留的金纳米花（AuNF）修饰电极来捕获目标，并使用适配体标记的金纳米颗粒/Cu-MOF（AuNPs/Cu-MOF）缀合物来产生敏感信号，获得了一种有效的夹心传感器，用于检测Aβ低聚物。 AuNPs/Cu-MOF缀合物独特的理化性质，如高电化学活性和大比表面积，赋予传感器优异的分析性能。设计的适体传感器具有1 nM至2 μM的宽线性范围，线性相关系数为0.996，检测Aβ寡聚体的检测限相对较低为0.45 nM。对于人工脑脊液中Aβ寡聚物的评价，高回收率和准确性表明了可行性，这将为阿尔茨海默病的早期诊断提供有价值的信息。此外，所提出的基于多功能MOF的电化学适体传感器将为传感其他分析物提供通用策略。
In this study, we developed a novel and facile electrochemical aptasensor for beta-amyloid (A beta) oligomer detection based on metal-organic frameworks (MOFs) as signal probes. By using an aptamer-tethered gold nanoflower (AuNF) modified electrode for target capture and aptamer-tagged gold nanoparticles/Cu-MOF (AuNPs/Cu-MOF) conjugates for sensitive signal generation, an effective sandwich sensor was obtained for the detection of A beta oligomers. The unique physico-chemical properties of AuNPs/Cu-MOF conjugates, such as high electrochemical activity and large surface area, endow the sensor with excellent analytical performances. The designed aptasensor exhibits a wide linear range from 1 nM to 2 mu M with a linear correlation coefficient of 0.996 and a relatively low detection limit of 0.45 nM for the detection of A beta oligomers. For the evaluation of A beta oligomers in artificial cerebrospinal fluid, the high recoveries and accuracy indicate the feasibility, which will provide valuable information for the early diagnosis of Alzheimer's disease. Moreover, the proposed electrochemical aptasensor based on multifunctional MOFs will provide a general strategy for sensing other analytes.