Non-coding telomeric and subtelomeric transcripts are differentially regulated by telomeric and heterochromatin assembly factors in fission yeast.
Non-coding telomeric and subtelomeric transcripts are differentially regulated by telomeric and heterochromatin assembly factors in fission yeast.
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DOI：
10.1093/nar/gkr1155
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发表时间：
2012-04
影响因子：
14.9
通讯作者：
Cooper JP
中科院分区：
文献类型：
--
作者：
Greenwood J;Cooper JP
虽然含有端粒重复序列的非编码RNA已在多种真核生物中被鉴定，但其生物学作用尚不清楚。我们已经在分裂酵母中发现了端粒转录本，这是一种将精确的基因操纵与端粒非常相似的模型系统结合在一起的模型系统。像人类和萌芽酵母一样，裂解酵母含有一组端粒RNA分子，其中含有从亚端粒转录到端粒的富含G的端粒重复序列。此外，我们检测到大量富含C的端粒RNA，其外观独立于依赖RNA的RNA聚合酶，表明端粒重复本身作为启动子位置；也存在多个不同的亚端粒RNA。这些转录本的调控依赖于端粒相关蛋白Taz1和Rap1，因为taz1+或Rap1+的缺失会导致含有端粒重复序列的转录本和亚端粒转录本的水平增加。相反，异染色质蛋白Swi6或CLR4或端粒酶调节因子Rif1的缺失会导致端粒下RNA水平升高，而含有端粒重复序列的转录水平仍然受到抑制。再加上围绕裂解酵母中端粒和异染色质因子功能的大量知识，这些体内分析为Terra在端粒功能中的作用提出了可测试的模型。
While telomere repeat-containing non-coding RNA has been identified in a variety of eukaryotes, its biological role is not yet clear. We have identified telomeric transcripts in fission yeast, a model system that combines precise genetic manipulability with telomeres remarkably similar to those of human. Like human and budding yeast, fission yeast harbours a population of telomeric RNA molecules containing G-rich telomeric repeats transcribed from the subtelomere to the telomere. In addition, we detect substantial levels of C-rich telomeric RNA whose appearance is independent of the RNA-dependent RNA polymerase, suggesting that the telomere repeats themselves serve as promoter sites; multiple distinct subtelomeric RNAs are also present. The regulation of these transcripts depends on the telomere-associated proteins Taz1 and Rap1, as deletion of taz1+ or rap1+ leads to increased levels of both telomere repeat-containing and subtelomeric transcripts. In contrast, loss of the heterochromatin proteins Swi6 or Clr4 or the telomerase regulator Rif1 results in elevated subtelomeric RNA levels while telomere-repeat containing transcript levels remain repressed. Coupled with the large body of knowledge surrounding the functions of telomeric and heterochromatin factors in fission yeast, these in vivo analyses suggest testable models for the roles of TERRA in telomere function.
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影响因子：
4.5
作者：
Nergadze, Solomon G.;Farnung, Benjamin O.;Azzalin, Claus M.
通讯作者：
Azzalin, Claus M.
影响因子：
2.6
作者：
Sato, M;Dhut, S;Toda, T
通讯作者：
Toda, T
影响因子：
5.3
作者：
Caslini, Corrado;Connelly, James A.;Hess, Jay L.
通讯作者：
Hess, Jay L.
DOI：
10.1073/pnas.232688099
发表时间：
2003-01-07
影响因子：
11.1
作者：
Hall, IM;Noma, K;Grewal, SIS
通讯作者：
Grewal, SIS
影响因子：
56.9
作者：
Azzalin, Claus M.;Reichenbach, Patrick;Lingner, Joachim
通讯作者：
Lingner, Joachim