Inhibitory effects of cis- and trans-isomers of 3,5-dihydroxystilbene on the activity of mushroom tyrosinase.
Inhibitory effects of cis- and trans-isomers of 3,5-dihydroxystilbene on the activity of mushroom tyrosinase.
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DOI：
10.1016/j.bbrc.2005.12.229
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发表时间：
2006-04
影响因子：
3.1
通讯作者：
Kang-Kang Song-Kang;Hao Huang;Peng Han;Chun-Le Zhang;Yan Shi;Qing-Xi Chen
中科院分区：
文献类型：
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作者：
Kang-Kang Song-Kang;Hao Huang;Peng Han;Chun-Le Zhang;Yan Shi;Qing-Xi Chen
研究了3，5-二羟基二苯乙烯顺式和反式异构体对蘑菇酪氨酸酶活性的影响。结果表明，3，5-二羟基二苯乙烯的顺式和反式异构体均能抑制该酶的活力，且抑制作用是可逆的。IC_（50）值分别为0.405±0.013和0.705±0.017mM。动力学分析表明，顺-3，5-二羟基二苯乙烯和反-3，5-二羟基二苯乙烯对该酶活力的抑制属于竞争型，抑制常数KI分别为0.232±0.015和0.395±0.020mM。本研究比较了顺式-3，5-二羟基二苯乙烯和反式-3，5-二羟基二苯乙烯对蘑菇酪氨酸酶的抑制作用。顺式异构体的抑制能力强于相应的反式异构体。然而，反式-3，5-二羟基芪比其相应的顺式化合物使用得更频繁。本研究为设计合成新型酪氨酸酶抑制剂提供了参考。此外，它可以提高二苯乙烯类化合物在食品防腐和脱色领域的应用。
The effects of cis- and trans-isomers of 3,5-dihydroxystilbene on the activity of mushroom tyrosinase have been studied. The results show that both cis- and trans-isomers of 3,5-dihydroxystilbene can inhibit the diphenolase activity of the enzyme and the inhibition type was reversible. The IC50values were estimated as 0.405±0.013 and 0.705±0.017mM, respectively. Kinetic analysis showed that the inhibition of cis-3,5-dihydroxystilbene and trans-3,5-dihydroxystilbene on the diphenolase activity of the enzyme belonged to competitive type, and the inhibition constants (KI) were determined to be 0.232±0.015 and 0.395±0.020mM, respectively. In this investigation, the inhibitory effects of cis-3,5-dihydroxystilbene and trans-3,5-dihydroxystilbene on the diphenolase activity of mushroom tyrosinase were compared. The inhibitory capacity of cis-isomer was stronger than that of corresponding trans-isomer. Nevertheless, the trans-3,5-dihydroxystilbene was used more frequently than its corresponding cis-form compound. This research may offer some references for designing and synthesizing some novel and effective tyrosinase inhibitors. Furthermore, it may improve the use of stilbenes on the field of food preservation and depigmentation.