Self-Organizing Actomyosin Patterns on the Cell Cortex at Epithelial Cell-Cell Junctions
Self-Organizing Actomyosin Patterns on the Cell Cortex at Epithelial Cell-Cell Junctions
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DOI：
10.1016/j.bpj.2014.10.045
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发表时间：
2014-12-02
影响因子：
3.4
通讯作者：
Neufeld, Zoltan
中科院分区：
文献类型：
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作者：
Moore, Thomas;Wu, Selwin K.;Neufeld, Zoltan
肌动球蛋白的行为决定了在粘附细胞之间的界面上发现的形态学上不同的收缩模式。一种这样的模式被发现在上皮细胞中的细胞-细胞连接的顶端区域（粘附小带），其中粘附分子E-钙粘蛋白的簇以静态模式集中。同时，E-cadherin集群整个横向细胞-细胞接触显示动态运动的平面上的连接。为了深入了解确定这些动态结构的性质和组织的原则，我们通过将2D肌动球蛋白细胞皮层建模为活性流体介质来分析这种行为。数值模拟表明，肌动蛋白丝的稳定性影响系统的空间结构和动力学。我们发现，除了静态图灵型模式，持久的动态行为发生在很大范围的参数。在2D模型中，机械应力依赖性肌动蛋白的故障被证明产生一个不断变化的网络肌动蛋白桥，而与一个恒定的故障率，更孤立的肌动球蛋白的集群往往形成。该模型定性地再现了在上皮细胞之间的连接处实验观察到的动态和稳定模式。
The behavior of actomyosin critically determines morphologically distinct patterns of contractility found at the interface between adherent cells. One such pattern is found at the apical region (zonula adherens) of cell-cell junctions in epithelia, where clusters of the adhesion molecule E-cadherin concentrate in a static pattern. Meanwhile, E-cadherin clusters throughout lateral cell-cell contacts display dynamic movements in the plane of the junctions. To gain insight into the principles that determine the nature and organization of these dynamic structures, we analyze this behavior by modeling the 2D actomyosin cell cortex as an active fluid medium. The numerical simulations show that the stability of the actin filaments influences the spatial structure and dynamics of the system. We find that in addition to static Turing-type patterns, persistent dynamic behavior occurs in a wide range of parameters. In the 2D model, mechanical stress-dependent actin breakdown is shown to produce a continuously changing network of actin bridges, whereas with a constant breakdown rate, more isolated clusters of actomyosin tend to form. The model qualitatively reproduces the dynamic and stable patterns experimentally observed at the junctions between epithelial cells.