Induction of gene expression in sheepshead minnows (Cyprinodon variegatus) treated with 17beta-estradiol, diethylstilbestrol, or ethinylestradiol: the use of mRNA fingerprints as an indicator of gene regulation.
Induction of gene expression in sheepshead minnows (Cyprinodon variegatus) treated with 17beta-estradiol, diethylstilbestrol, or ethinylestradiol: the use of mRNA fingerprints as an indicator of gene regulation.
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用 17β-雌二醇、己烯雌酚或炔雌醇处理的羊头鲦鱼 (Cyprinodon variegatus) 中基因表达的诱导：使用 mRNA 指纹作为基因调控的指标。
DOI：
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发表时间：
2001
影响因子：
2.7
通讯作者：
L. Folmar
中科院分区：
文献类型：
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作者：
N. Denslow;C. Bowman;Ronald J. Ferguson;H.Stephen Lee;M. J. Hemmer;L. Folmar
最近对激素活性环境污染物的兴趣激发了人们寻找敏感方法来测量它们对野生动物的影响的动力。研究人员开发了一种基于分子的检测方法，用于测量羊头鲦鱼（Cyprinodon variegatus）体内暴露于天然和药物雌激素17 -雌二醇、炔雌醇和己烯雌酚的基因表达诱导。该方法采用差异显示逆转录酶聚合酶链反应法比较对照鱼和雌激素暴露鱼个体mrna的表达。用这种方法分离了48个差异表达的cdna，包括卵黄膜蛋白和卵黄原蛋白的cdna。注射雌二醇药理学剂量（5 mg/kg）的鱼的mRNA表达模式与持续暴露于212 ng /l雌二醇的鱼的mRNA表达模式相同。此外，在雌二醇处理过的鱼中观察到的cDNA“指纹”模式也与连续暴露于192 ng乙炔雌二醇/升和200 ng己烯雌酚/升的名义浓度的水中的鱼所获得的dna“指纹”模式相匹配。研究结果表明，暴露于多种天然和人为雌激素中，基因的表达模式有所上调，并表明这种方法可能对研究环境污染物对其他内分泌介导的生殖、生长和发育途径的潜在影响有价值。
The recent interest in hormonally active environmental contaminants has sparked a drive to find sensitive methods to measure their effects on wildlife. A molecular-based assay has been developed to measure the induction of gene expression in sheepshead minnows (Cyprinodon variegatus) exposed in vivo to the natural and pharmaceutical estrogens 17beta-estradiol, ethinylestradiol, and diethylstilbestrol. This method used differential display reverse transcriptase polymerase chain reaction assays to compare the expression of individual mRNAs from control and estrogen-exposed fish. Forty-eight differentially expressed cDNAs were isolated by this method, including cDNAs for vitelline envelope proteins and vitellogenin. The mRNA expression patterns for fish injected with a pharmacological dose of estradiol (5 mg/kg) were identical to those obtained in fish receiving constant aqueous exposure to 212 ng estradiol/liter. Further, the cDNA "fingerprint" pattern observed in the estradiol-treated fish also matched that obtained in fish receiving continuous-flow aqueous exposures to 192 ng ethinyl estradiol/liter and a nominal concentration of 200 ng diethylstilbestrol/liter. The results demonstrate a characteristic expression pattern for genes upregulated by exposure to a variety of natural and anthropogenic estrogens and suggest this approach may be valuable to examine the potential effects of environmental contaminants on other endocrine-mediated pathways of reproduction, growth, and development.
影响因子：
10.4
作者：
Walsh LP;McCormick C;Martin C;Stocco DM
通讯作者：
Stocco DM
雌性 BALB/cCrgl 小鼠新生儿暴露于不同剂量的己烯雌酚后，生殖道和乳腺的组织学和类固醇受体水平的长期变化。
影响因子：
11.2
作者：
Bern,HA;Edery,M;Mills,KT;Kohrman,AF;Mori,T;Larson,L
通讯作者：
Larson,L