Overexpression of CYP6ER1 associated with clothianidin resistance in Nilaparvata lugens (Stal)
Overexpression of CYP6ER1 associated with clothianidin resistance in Nilaparvata lugens (Stal)
复制标题
CYP6ER1 过度表达与褐飞虱 (Nilaparvata lugens) 中噻虫胺耐药性相关 (Stàl)
DOI：
10.1016/j.pestbp.2018.12.008
复制
发表时间：
2019-02-01
影响因子：
4.7
通讯作者：
Li, Jianhong
中科院分区：
文献类型：
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作者：
Jin, Ruoheng;Mao, Kaikai;Li, Jianhong
褐飞虱（Nilaparvata lugens（Stal））是亚洲最重要的水稻害虫之一，对包括烟碱类杀虫剂在内的多种杀虫剂产生了抗药性。在这项研究中，一个N。建立了褐飞虱噻虫胺抗性品系和敏感品系，并对褐飞虱噻虫胺的抗性机制进行了初步探讨。lugens到噻虫胺的转化。交叉抗性研究表明，该品系对大多数烟碱类杀虫剂均表现出交叉抗性，尤其是烯啶虫胺（99.19倍）和呋虫胺（77.68倍），而对毒死蜱（1.79倍）无交叉抗性。协同作用试验和解毒酶的活性进行，我们发现，细胞色素P450赋予噻虫胺抗性。qRT-PCR结果显示，与CLS株相比，CYP 6 ER 1和CYP 6AY 1在P450基因中的表达量显著增加。此外，通过RNA干扰敲低CYP 6 ER 1显著增加噻虫胺对N. lugens。这些数据表明，CYP 6 ER 1的过表达可能是N. lugens。本研究结果将有助于改进褐飞虱抗性管理策略的设计。
The brown planthopper, Nilaparvata lugens (Stal), is one of the most economically important rice pests in Asia and has become resistant to various kinds of insecticides, including neonicotinoid insecticides. In this study, an N. lugens clothianidin-resistant (CLR) strain and a susceptible (CLS) strain were established, and the potential resistance mechanisms of N. lugens to clothianidin were elucidated. The cross-resistance studies showed that the clothianidin-resistant strain exhibited cross-resistance to most neonicotinoid insecticides, especially nitenpyram (99.19-fold) and dinotefuran (77.68-fold), while there was no cross-resistance to chlorpyrifos (1.79-fold). The synergism assays and the activities of the detoxification enzymes were performed, and we found that a cytochrome P450 conferred the clothianidin resistance. Two P450 genes (CYP6ER1 and CYP6AY1) were found to be significantly overexpressed in the CLR strain compared with the CLS strain based on qRT-PCR. In addition, the knockdown of CYP6ER1 by RNA interference dramatically increased the toxicity of clothianidin against N. lugens. These data demonstrated that the overexpression of CYP6ER1 could contribute to clothianidin resistance in N. lugens. Our findings will help to improve the design of effective resistance management strategies to control brown planthoppers.