Mass spectrometry for structural analysis and quantification of the Major Urinary Proteins of the house mouse
Mass spectrometry for structural analysis and quantification of the Major Urinary Proteins of the house mouse
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DOI：
10.1016/j.ijms.2015.07.026
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发表时间：
2015-11-30
影响因子：
1.8
通讯作者：
Woolerton, Yvonne E.
中科院分区：
文献类型：
--
作者：
Beynon, Robert J.;Armstrong, Stuart D.;Woolerton, Yvonne E.
家鼠（Mus musculus luticus）的主要尿蛋白（MUP）是18-19 kDa的β-桶脂质运载蛋白，参与个体之间的化学通讯。它们中的许多在尿液中排泄，在那里它们发挥多种作用，包括编码主人身份和运输，以及缓慢释放结合的挥发性信息素。其中之一，darcin，本身就是一种信息素，并诱导对气味标记所有者的身份和位置的长期记忆。我们已经表明，完整的蛋白质，其离子迁移率行为的质谱分析，是能够解剖这类蛋白质的成员之间的细微结构差异。此外，完整蛋白质的质谱分析可以有助于MUP的分子表型分析。然而，在允许相对量化的同时，各个MUP的电离倾向或气相特性可能会损害绝对量化。为了解决MOP表达的绝对定量的挑战，我们设计并构建了由内肽酶LysC肽构建的QconCAT，其能够定量实验室动物品系中发现的MUP和来自野生捕获个体的一些MUP。(C)2015年由Elsevier B. V.出版
The Major Urinary Proteins (MUPs) of the house mouse, Mus musculus domesticus, are 18-19 kDa beta-barrel lipocalins that are involved in chemical communication between individuals. Many of them are excreted in urine where they play multiple roles, including coding of owner identity and transport, and slow release of bound volatile pheromones. One of them, darcin, is a pheromone in its own right and induces long-term memory for the identity and location of the scent mark owner. We have shown that mass spectrometric analysis of intact proteins, and their ion mobility behaviour, is capable of dissecting subtle structural differences between the members of this class of proteins. Moreover, mass spectrometric analysis of the intact proteins can contribute towards molecular phenotyping of MUPs. However, whilst allowing relative quantification, the ionisation propensity or gas phase properties of the individual MUPs may compromise absolute quantification. To solve the challenge of absolute quantification of MOP expression, we have designed and constructed a QconCAT built from endopeptidase LysC peptides that is capable of quantifying MUPs found in laboratory animal strains and some MUPs from wild caught individuals. (C) 2015 Published by Elsevier B.V.