Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24
Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24
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DOI：
10.1006/geno.1998.5650
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发表时间：
1999-02-01
期刊：
影响因子：
4.4
通讯作者：
Liu, WG
中科院分区：
文献类型：
--
作者：
Mai, M;Qian, CP;Liu, WG
传导蛋白或Axil是一种Axin同源物，在Wnt信号通路中β-连环蛋白稳定性的调节中起重要作用。为了便于人类基因的分子分析，我们分离了人类同源物AXIN 2。该cDNA包含一个2529 bp的开放阅读框，编码843个氨基酸。与大鼠和小鼠同源物相比，AXIN 2显示出总体89%的氨基酸同一性。该蛋白中的几个功能结构域高度保守，包括GRS（95.9%）、GSK-3 β（96.3%）、Dsh（98%）和β-连环蛋白（89.9%）结构域。放射杂交定位AXIN 2基因定位于人类染色体17 q23-q24，这是一个在乳腺癌、神经母细胞瘤和其他肿瘤中经常出现杂合性丢失的区域。因此，人AXIN 2是涉及多种肿瘤类型的非常强的候选者。(C)北京：科学出版社.
Conductin or Axil, an Axin homolog, plays an important role in the regulation of beta-catenin stability in the Wnt signaling pathway. To facilitate the molecular analysis of the human gene, we isolated the human homolog, AXIN2. The cDNA contains a 2529-bp open reading frame and encodes a putative protein of 843 amino acids. Compared with rat and mouse homologs, AXIN2 shows an overall 89% amino acid identity. Several functional domains in this protein are highly conserved including the GRS (95.9%), GSK-3 beta (96.3%), Dsh (98%), and beta-catenin (89.9%) domains. Radiation hybrid mapping localized the AXIN2 gene to human chromosome 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Human AXIN2 is thus a very strong candidate involved in multiple tumor types. (C) 1999 Academic Press.