The core ubiquitin system of mandarin fish, Siniperca chuatsi, can be utilized by infectious spleen and kidney necrosis virus
The core ubiquitin system of mandarin fish, Siniperca chuatsi, can be utilized by infectious spleen and kidney necrosis virus
复制标题
鳜鱼的核心泛素系统可被传染性脾肾坏死病毒利用
DOI：
10.1016/j.fsi.2017.09.017
复制
发表时间：
2017-11-01
影响因子：
4.7
通讯作者：
Xie, Junfeng
中科院分区：
文献类型：
--
作者：
Huang, Runqing;Zhang, Jing;Xie, Junfeng
泛素化过程调节各种细胞过程。鱼类的泛素-蛋白酶体系统（UPS）是水生病毒免疫逃逸的靶点，在机体产生天然免疫和适应性免疫应答中起重要作用。我们克隆了鳜鱼的泛素激活酶（ScE 1）、泛素结合酶（ScE 2）和HECT型泛素连接酶（ScE 3）三个基因，并对其进行了鉴定。这些基因在所有组织中表达，在血液中观察到最高水平。在感染传染性脾肾坏死病毒（ISKNV）的鳜鱼鱼苗细胞中，3个基因的表达水平在体外几乎一致，且在早期表达上调，晚期表达下调。在感染ISKNV的桂鱼血液中，它们在体内的表达均下调，但在不同的时间点达到峰值，表明病毒感染抑制UPS。此外，这些重组蛋白被确定为在体外泛素化测定中良好地起作用。此外，ScE 1和ScE 2可被ISKNV编码的四种RING型病毒E3（vE 3）利用。vE 3的体外活性强于ScE 3，表明ISKNV可能通过E3活性竞争和表达调控劫持鱼类UPS。本研究探讨了桂鱼UPS的功能及其对虹彩病毒感染的反应，为更好地了解病毒与宿主的相互作用以及ISKNV逃避宿主免疫反应的机制提供了新的思路。(C)2017爱思唯尔有限公司版权所有
The process of ubiquitination regulates various cellular processes. The ubiquitin-proteasome system (UPS) in fish, which is important for the generation of innate and adaptive immune responses to pathogens, is the target of aquatic viruses to achieve immune evasion. We cloned and characterized three genes, namely, a ubiquitin-activating enzyme (ScE1), a ubiquitin-conjugating enzyme (ScE2), and a HECT-type ubiquitin ligase (ScE3) of mandarin fish Siniperca chuatsi. The genes were expressed in all tissues and the highest levels were observed in the blood. In infectious spleen and kidney necrosis virus (ISKNV)-infected mandarin fish fry cells, the expression levels of the three genes in vitro were almost identical, and upregulated during the early stage and downregulated at the late stage. In the blood of ISKNV-infected mandarin fish, their expressions in vivo were downregulated equally although peaking at different timepoints, indicating the suppression of UPS by viral infection. Furthermore, these recombinant proteins were determined to function well in ubiquitination assays in vitro. Moreover, ScE1 and ScE2 can be utilized by four RING-type viral E3s (vE3s) that are encoded by ISKNV. The in vitro activity of vE3 was stronger than that of ScE3, suggesting that the fish UPS may be hijacked by ISKNV via E3 activity competition and expression modulation. The present study investigated the function of mandarin fish UPS as well as its response to iridovirus infection, providing insights to better understand the virus-host interactions and the mechanism of ISKNV in evading host immune responses. (C) 2017 Elsevier Ltd. All rights reserved.