Application of DNA microarray technology to gerontological studies.
Application of DNA microarray technology to gerontological studies.
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DOI：
10.1007/978-1-62703-556-9_19
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发表时间：
2013
影响因子：
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通讯作者：
Kiyoshi Masuda;Y. Kuwano;K. Nishida;K. Rokutan
中科院分区：
文献类型：
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作者：
Kiyoshi Masuda;Y. Kuwano;K. Nishida;K. Rokutan
基因表达模式在衰老和年龄相关事件中发生巨大变化。 DNA微阵列现已被认为是分子生物学中有用的装置，并广泛用于识别衰老的分子机制以及用于治疗与年龄相关的疾病的药物的生物学效应。最近，众多技术优势导致 DNA 微阵列和基于微阵列的技术的发展，揭示了基因组修饰和所有转录活性。在这里，我们展示了我们实验室目前使用的处理寡核苷酸微阵列和 miRNA 微阵列的分步方法。此外，我们介绍了核糖核蛋白 [RNP] 免疫沉淀和微阵列分析（RIP 芯片）的方案，该方案揭示了与年龄相关的 RNA 结合蛋白的靶 mRNA。
Gene expression patterns change dramatically in aging and age-related events. The DNA microarray is now recognized as a useful device in molecular biology and widely used to identify the molecular mechanisms of aging and the biological effects of drugs for therapeutic purpose in age-related diseases. Recently, numerous technological advantages have led to the evolution of DNA microarrays and microarray-based techniques, revealing the genomic modification and all transcriptional activity. Here, we show the step-by-step methods currently used in our lab to handling the oligonucleotide microarray and miRNA microarray. Moreover, we introduce the protocols of ribonucleoprotein [RNP] immunoprecipitation followed by microarray analysis (RIP-chip) which reveal the target mRNA of age-related RNA-binding proteins.