Interactions between primed and unprimed cells in the regulation of in vitro antibody responses. I. Role of “plasma cells” as inducers of suppression
Interactions between primed and unprimed cells in the regulation of in vitro antibody responses. I. Role of “plasma cells” as inducers of suppression
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已引发和未引发的细胞在体外抗体反应调节中的相互作用 I.“浆细胞”作为抑制诱导剂的作用。
DOI：
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发表时间：
1982
影响因子：
5.4
通讯作者：
C. Calkins
中科院分区：
文献类型：
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作者：
C. Calkins
已证明特异性免疫抑制在培养物中通过引发和未引发的 T 细胞亚群的相互作用而被激活。免疫后8天在脾脏中发现涉及的引发细胞，但在淋巴结或胸腺细胞群中没有发现。当在与未分离的未引发的细胞一起培养之前，通过尼龙毛传代或抗 Thy-1 加补体 (C) 处理对引发的脾细胞进行分级时，只有在共培养物中存在引发的 B 细胞时才能检测到抑制。用抗PC处理引发的脾细胞。 1（浆细胞特异性抗血清）加 C 消除了它们与未分离的或富含 T 细胞的未引发细胞群体合作抑制共培养物抗体反应的能力。这些数据与以下假设一致：分泌抗体的浆细胞会激活先前未接触过抗原的细胞群中的抑制性 T 细胞前体。
Specific immune suppression has been shown to be activated in culture by the interaction of primed and unprimed T cell subsets. The primed cell involved is found 8 days after immunization in spleen but not in lymph node or thymus cell populations. When the primed spleen cells were fractionated by nylon wool passage or anti‐Thy‐1 plus complement (C) treatment, prior to culture with unseparated unprimed cells, suppression was detectable only with primed B cells present in the co‐cultures. Treatment of the primed spleen cells with anti‐PC. 1 (an antiserum specific for plasma cells) plus C eliminated their ability to cooperate with either unseparated or T cell‐enriched populations of unprimed cells in suppressing the antibody response of the co‐cultures. These data are consistent with the hypothesis that antibody‐secreting plasma cells activate suppressor T cell precursors in cell populations not previously exposed to antigen.