Immunohistochemical localization of amyloid β-protein with amino-terminal aspartate in the cerebral cortex of patients with Alzheimer's disease
Immunohistochemical localization of amyloid β-protein with amino-terminal aspartate in the cerebral cortex of patients with Alzheimer's disease
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DOI：
10.1016/s0006-8993(98)01366-3
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发表时间：
1999-03-27
期刊：
影响因子：
2.9
通讯作者：
Mori, H
中科院分区：
文献类型：
--
作者：
Arai, T;Akiyama, H;Mori, H
我们采用免疫组织化学方法研究了氨基末端为天冬氨酸（N1[D]）的β -淀粉样蛋白（Aβ）在阿尔茨海默病、弥漫性路易体病和唐氏综合征患者大脑中的定位。使用一种识别Aβ中间部分的单克隆抗体4G8作为参照抗体来标记所有的Aβ沉积物。用抗Aβ（N1[D]）和4G8进行双重染色显示，海马下托和新皮质深层的Aβ沉积物常常缺乏N1[D]免疫反应性，这表明这些沉积物中Aβ的氨基末端被截断。新皮质浅层和海马下托前小锥体细胞层的Aβ沉积物总是含有带N1[D]的Aβ。这种从N1[D]开始的Aβ分布在区域和层状上的差异表明，一些局部因素影响着大脑中沉积的Aβ的氨基末端加工过程。（C）1999年，爱思唯尔科学出版社。版权所有。
We investigated immunohistochemically the localization of amyloid beta-protein (A beta) with amino-terminal aspartate (N1[D]) in brains of patients with Alzheimer's disease, diffuse Lewy body disease and Down's syndrome. A monoclonal antibody, 4G8, which recognizes the middle portion of A beta, was used as a reference antibody to label the total A beta deposits. Double staining with anti-A beta(N1[D]) and 4G8 revealed that A beta deposits in the subiculum and the neocortical deep layers often lacked N1[D] immunoreactivity, indicating N-terminal truncation of A beta in these deposits. A beta deposits in the neocortical superficial layers and the presubicular parvopyramidal layer always contained A beta with N1[D]. Such regional as well as laminnr differences in the distribution of A beta beginning at N1[D] suggest that some local factors influence N-terminal processing of A beta deposited in the brain. (C) 1999 Elsevier Science B.V. All rights reserved.