Enzyme-free translation of DNA into sequence-defined synthetic polymers structurally unrelated to nucleic acids.
Enzyme-free translation of DNA into sequence-defined synthetic polymers structurally unrelated to nucleic acids.
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DNA序列翻译成相应的生物聚合物使得生命大分子的产生、功能和进化成为可能。相比之下，产生具有类似控制水平的序列限定的合成聚合物的方法仍然难以捉摸。在这里，我们报告的DNA模板的翻译系统，使酶的DNA模板到序列定义的合成聚合物，没有必要的结构关系的核酸的无翻译的发展。我们证明了效率，序列特异性，和通用性的翻译系统，通过寡聚化的积木，包括聚乙二醇（PEG），α-（d）-肽，和β-肽在DNA编程的方式。使用该策略从DNA模板翻译具有26 kDa分子量的序列限定的合成聚合物，其含有16个连续偶联的结构单元和90个密集官能化的β-氨基酸残基。我们将这里开发的DNA模板化翻译系统整合成一个完整的翻译循环，编码序列复制，模板再生和重新翻译，适合于体外迭代选择与核酸结构无关的功能序列定义的合成聚合物。
The translation of DNA sequences into corresponding biopolymers enables the production, function, and evolution of the macromolecules of life. In contrast, methods to generate sequence-defined synthetic polymers with similar levels of control have remained elusive. Here we report the development of a DNA-templated translation system that enables the enzyme-free translation of DNA templates into sequence-defined synthetic polymers that have no necessary structural relationship with nucleic acids. We demonstrate the efficiency, sequence-specificity, and generality of this translation system by oligomerizing building blocks including polyethylene glycol (PEG), α-(d)-peptides, and β-peptides in a DNA-programmed manner. Sequence-defined synthetic polymers with molecular weights of 26 kDa containing 16 consecutively coupled building blocks and 90 densely functionalized β-amino acid residues were translated from DNA templates using this strategy. We integrated the DNA-templated translation system developed here into a complete cycle of translation, coding sequence replication, template regeneration, and re-translation suitable for the iterated in vitro selection of functional sequence-defined synthetic polymers unrelated in structure to nucleic acids.