A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion
A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion
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DOI：
10.1016/j.jgg.2011.02.004
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发表时间：
2011-03-01
影响因子：
5.9
通讯作者：
Tsugane, Kazuo
中科院分区：
文献类型：
--
作者：
Hayashi-Tsugane, Mika;Maekawa, Masahiko;Tsugane, Kazuo
我们分离了一个水稻隐性突变体，命名为不确定生长（indeindegrowing），它在营养期表现出匍匐和明显的异时表型，茎长而卷曲。粗略定位和随后的分子表征显示，该突变体携带一个大缺失，对应于Nipponbare基因组103 kb的区域，包含3号染色体上的9个注释基因。在这些被注释的基因中，编码DELLA蛋白的SLR1基因是唯一一个功能明确的基因，它的零突变是由无内含子的SLR1基因中间的单碱基缺失引起的，赋予了一个细长的表型，与ing突变表型非常相似。ing突变表型的主要原因是SLR1基因的缺失，而ing突变似乎是第一个具有整个SLR1序列缺失的特征突变。我们的研究结果还表明，103 kb的缺失区域不包含一个不可或缺的基因，其功能障碍必然导致致死表型。
We have isolated a recessive rice mutant, designated as indeterminate growth (ing), which displays creeping and apparent heterochronic phenotypes in the vegetative period with lanky and winding culms. Rough mapping and subsequent molecular characterization revealed that the ing mutant carries a large deletion, which corresponds to a 103 kb region in the Nipponbare genome, containing nine annotated genes on chromosome 3. Of these annotated genes, the SLR1 gene encoding a DELLA protein is the only one that is well characterized in its function, and its null mutation, which is caused by a single base deletion in the middle of the intronless SLR1 gene, confers a slender phenotype that bears close resemblance to the ing mutant phenotype. The primary cause of the ing mutant phenotype is the deletion of the SLR1 gene, and the ing mutant appears to be the first characterized mutant having the entire SLR1 sequence deleted. Our results also suggest that the deleted region of 103 kb does not contain an indispensable gene, whose dysfunction must result in a lethal phenotype.