MUCUS GLYCOPROTEIN CONTENT OF HUMAN CHOLESTEROL GALLSTONES
MUCUS GLYCOPROTEIN CONTENT OF HUMAN CHOLESTEROL GALLSTONES
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DOI：
10.1159/000199410
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发表时间：
1987-01-01
期刊：
影响因子：
3.2
通讯作者：
FOSTER, SNE
中科院分区：
文献类型：
--
作者：
PEARSON, JP;FOSTER, SNE
从接受胆囊切除术的患者中获得胆固醇结石，并提取粘液糖蛋白。通过Sepharose 4 B凝胶过滤将胆汁粘液糖蛋白与其他污染物分离，并将PAS染色排除体积用于估计胆囊结石的粘液糖蛋白含量。糖蛋白的己糖胺和唾液酸分析表明其在组成上与来自胆汁的人粘液糖蛋白相似。所分析的9个结石的粘液糖蛋白含量分别在0.75和2.3 mg之间变化（3倍变化）（1.27 ± 0.01 mg）。0.16 mg，hivin.x .+-。SEM），而结石重量在0.076和5.885 g之间变化（77倍变化）（1.27 ± 0.001 g）。0.63 g.hivin.x .+-。SEM）。当提取平均重量为47 mg的较小结石时，仅分离出1.73 mg糖蛋白，平均为0.01 mg/结石。在Sepharose 2B上通过凝胶过滤对粘液糖蛋白进行分析，结果显示，与胆汁中的粘液糖蛋白一样，大部分糖蛋白被排除在外。这些结果与胆汁粘液糖蛋白参与胆结石形成的初始阶段，但不参与随后的生长是一致的。
Cholesterol gallstones were obtained from patients undergoing cholecystectomy and the mucus glycoprotein extracted. The biliary mucus glycoprotein was separated from other contaminants by Sepharose 4B gel filtration and the PAS staining excluded volume used to estimate mucus glycoprotein content of the gallstones. Hexosamine and sialic acid analysis of the glycoprotein indicated it was compositionally similar to the human mucus glycoprotein from bile. The mucus glycoprotein content of the nine stones analyzed individually varied between 0.75 and 2.3 mg (a 3-fold variation) (1.27 .+-. 0.16 mg, .hivin.x .+-. SEM), whereas stone weight varied between 0.076 and 5.885 g (a 77-fold variation) (1.27 .+-. 0.63 g, .hivin.x .+-. SEM). When a pool of smaller stones, average weight 47 mg, was extracted, only 1.73 mg of glycoprotein was isolated, an average of 0.01 mg/stone. Analysis of the mucus glycoprotein by gel filtration on Sepharose 2B showed the majority of the glycoprotein was excluded as is the case with the mucus glycoprotein in bile. These results are consistent with biliary mucus glycoprotein being involved in the initial stages of gallstone formation but not in subsequent growth.