Some facts concerning the nature and formation of axial core structures in spermatids of Gryllus domesticus
Some facts concerning the nature and formation of axial core structures in spermatids of Gryllus domesticus
复制标题
关于蟋蟀精子细胞轴核结构的性质和形成的一些事实
DOI：
10.1007/bf00326872
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发表时间：
2004
期刊：
影响因子：
1.6
通讯作者：
G. F. Meyer
中科院分区：
文献类型：
--
作者：
D. Wolstenholme;G. F. Meyer
对家蟋蟀活精细胞核的检查发现存在相同的结构：X染色体、圆形体和轴核结构，这些结构已通过电子显微镜观察得到描述。轴心结构和圆形体的外带由 100 Å 纤维组成，这些纤维与组成 X 染色体的纤维无法区分，并且通常与组成 X 染色体的纤维连续。甲醛固定材料的细胞化学检查结果进一步证实了轴心结构和圆体的外带是染色体，轴心结构和圆体含有RNA、DNA和碱性蛋白。乙酸-乙醇和冷乙醇固定都不能保留圆形体和轴核心结构，这表明蛋白质可能负责维持中央核心结构。在染色体元件相距约 1000 Å 的区域中，总是发现中央核心结构与浓缩染色质密切相关，这表明染色质浓缩的相对状态和浓缩区域之间的空间关系可能是中央核心形成的两个主要因素。然而，染色质浓缩状态的维持可能又取决于中央核心的完整性。
Examination of living spermatid nuclei of Gryllus domesticus has revealed the presence of the same structures, the X chromosome, the round body and the axial core structures, which have been described from electron microscopic observations. The outer ribbons of the axial core structures and the round body are composed of 100 Å fibres indiscernible from and often continuous with the fibres composing the X chromosome. That the outer ribbons of the axial core structures and the round body are chromosomal is further substantiated by the results of cytochemical examinations of formaldehyde fixed material which show that the axial core structures and the round body contain RNA, DNA and basic protein. Neither acetic acid-ethanol nor cold ethanol fixation preserve the round body and the axial core structures suggesting that a protein may be responsible for maintenance of the central core structure. The central core structures are always found in close association with condensed chromatin in regions where the chromosome elements are about 1000 Å apart, suggesting that the relative state of condensation of the chromatin and the spacial relationship between condensed regions may be two of the chief factors concerned in central core formation. Maintainance of the condensed state of the chromatin, however, may in turn depend upon central core integrity.