EFFECTS OF VOLATILE ANESTHETICS ON THE CIRCADIAN RHYTHMS OF RAT HIPPOCAMPAL ACETYLCHOLINE RELEASE AND LOCOMOTOR ACTIVITY
EFFECTS OF VOLATILE ANESTHETICS ON THE CIRCADIAN RHYTHMS OF RAT HIPPOCAMPAL ACETYLCHOLINE RELEASE AND LOCOMOTOR ACTIVITY
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DOI：
10.1016/j.neuroscience.2013.01.062
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发表时间：
2013-05-01
期刊：
影响因子：
3.3
通讯作者：
Kamiya, Y.
中科院分区：
文献类型：
--
作者：
Kikuchi, T.;Tan, H.;Kamiya, Y.
全身麻醉偶尔与术后并发症有关，如睡眠障碍、嗜睡或情绪改变。海马乙酰胆碱（ACh），其细胞外水平在大鼠的黑暗（活动）阶段增加，在光明（休息）阶段减少，被认为与运动活动有关，对学习和记忆至关重要。丙泊酚是一种静脉麻醉剂，已知可改变啮齿动物和人类生理参数的昼夜节律，包括运动活动和体温，而挥发性麻醉剂对昼夜节律的影响在很大程度上尚不清楚。本研究观察了异氟醚麻醉对大鼠海马ACh释放和自发活动的昼夜变化的影响。将大鼠分为3组：光相麻醉组（LA组）、暗相麻醉组（DA组）和对照组。将动物置于12 h光照/12 h黑暗环境中，在光照期（LA组）和黑暗期（DA组）中间用1.4%异氟醚麻醉4 h。在自由活动条件下，通过微透析和自发活动同时测量海马ACh。海马ACh释放和运动活动表现出明显的昼夜节律。在DA组，但不是在LA组，乙酰胆碱释放的昼夜变化显着干扰，并观察到超过2小时的阶段提前自发活动。海马ACh释放和自发活动之间存在显著相关性，即使在麻醉停止后，异氟烷麻醉也会破坏它。这项研究表明，海马ACh释放和自发活动的水平和昼夜节律对异氟醚麻醉更敏感时，它是在活动期给药。我们的研究结果表明，麻醉对昼夜节律的调节产生不同的影响，这取决于昼夜节律阶段。(C)2013年BRO。由爱思唯尔有限公司出版。保留所有权利。
General anesthesia is occasionally associated with postoperative complications such as sleep disorder, drowsiness, or mood alterations. Hippocampal acetylcholine (ACh), the extracellular level of which increases during the dark (active) phase and decreases during the light (rest) phase in rats, is thought to be associated with locomotor activity and be crucial for learning and memory. Propofol, an intravenous anesthetic, is known to shift the circadian rhythms of physiological parameters including locomotor activity and body temperature in both rodents and humans, while the effects of volatile anesthetics on the circadian rhythm largely remain unclear. The present study examined the effects of isoflurane anesthesia on the diurnal changes in hippocampal ACh release and locomotor activity in rats. Rats were divided into three groups: a light-phase anesthesia group (LA group), a dark-phase anesthesia group (DA group), and a control group. They were exposed to a 12-h light/12-h dark environment and anesthetized with 1.4% isoflurane for 4h during the middle of the light phase (LA group) and dark phase (DA group). Simultaneous measurement of hippocampal ACh by microdialysis and locomotor activity were done for 60 h under free-moving conditions. Hippocampal ACh release and locomotor activity showed a clear circadian rhythm. In the DA group, but not in the LA group, the diurnal variation in ACh release was significantly disturbed and a more than 2-h phase-advance in locomotor activity was observed. There was a significant correlation between hippocampal ACh release and locomotor activity, and isoflurane anesthesia disrupted it even after anesthesia was discontinued. This study revealed that the levels and circadian rhythms of hippocampal ACh release and locomotor activity were more sensitive to isoflurane anesthesia when it was administered during the active phase. Our findings suggest that anesthesia exerts differential effects on the regulation of circadian rhythms depending on the circadian phase. (C) 2013 BRO. Published by Elsevier Ltd. All rights reserved.