Developing high-affinity, oxygen-insensitive [NiFe]-hydrogenases as biocatalysts for energy conversion.
Developing high-affinity, oxygen-insensitive [NiFe]-hydrogenases as biocatalysts for energy conversion.
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DOI：
10.1042/bst20230120
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发表时间：
2023-10-31
影响因子：
3.9
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中科院分区：
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氢（H2）的裂解是一种能量产生过程，其对于生物系统和作为提供绿色能量的手段都是重要的。在生物学中，这种反应是由称为氢化酶的酶介导的，氢化酶利用复杂的镍和铁辅因子来分裂H2并将产生的电子转移到电子受体。这些[NiFe]-氢化酶作为燃料电池中的催化剂受到了相当大的关注，燃料电池利用H2产生电流。[NiFe]-氢化酶是目前在燃料电池中占主导地位的铂基催化剂的有前途的替代品，这是由于镍和铁的丰度，以及一些家族成员对包括一氧化碳在内的气体抑制的抗性，这些气体会迅速使铂基催化剂中毒。然而，大多数表征的[NiFe]-氢化酶被氧（O2）抑制，限制了它们的活性和稳定性。我们最近报道了分离和表征的[NiFe]-氢化酶Huc从耻垢分枝杆菌，这是不敏感的抑制O2和具有极高的亲和力，使其能够氧化H2在空气中低于大气浓度。这些特性使Huc成为开发酶基燃料电池（EBFC）的有希望的候选者，EBFC利用低浓度和不纯气体混合物中的H2。在这篇综述中，我们的目标是提供背景下使用Huc为此目的，通过讨论的优势[NiFe]-氢化酶作为催化剂和它们在燃料电池中的部署。我们还讨论了与使用[NiFe]-氢化酶用于此目的相关的挑战，以及如何克服这些挑战以开发可大规模部署的EBFC。
The splitting of hydrogen (H2) is an energy-yielding process, which is important for both biological systems and as a means of providing green energy. In biology, this reaction is mediated by enzymes called hydrogenases, which utilise complex nickel and iron cofactors to split H2 and transfer the resulting electrons to an electron-acceptor. These [NiFe]-hydrogenases have received considerable attention as catalysts in fuel cells, which utilise H2 to produce electrical current. [NiFe]-hydrogenases are a promising alternative to the platinum-based catalysts that currently predominate in fuel cells due to the abundance of nickel and iron, and the resistance of some family members to inhibition by gases, including carbon monoxide, which rapidly poison platinum-based catalysts. However, the majority of characterised [NiFe]-hydrogenases are inhibited by oxygen (O2), limiting their activity and stability. We recently reported the isolation and characterisation of the [NiFe]-hydrogenase Huc from Mycobacterium smegmatis, which is insensitive to inhibition by O2 and has an extremely high affinity, making it capable of oxidising H2 in air to below atmospheric concentrations. These properties make Huc a promising candidate for the development of enzyme-based fuel cells (EBFCs), which utilise H2 at low concentrations and in impure gas mixtures. In this review, we aim to provide context for the use of Huc for this purpose by discussing the advantages of [NiFe]-hydrogenases as catalysts and their deployment in fuel cells. We also address the challenges associated with using [NiFe]-hydrogenases for this purpose, and how these might be overcome to develop EBFCs that can be deployed at scale.
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5.2
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6.4
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6.4
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