Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus.
Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus.
复制标题
DOI：
10.1186/1743-422x-9-218
复制
发表时间：
2012-09-24
期刊：
影响因子：
4.8
通讯作者：
Fischer A
中科院分区：
文献类型：
--
作者：
Masembe C;Michuki G;Onyango M;Rumberia C;Norling M;Bishop RP;Djikeng A;Kemp SJ;Orth A;Skilton RA;Ståhl K;Fischer A
对猪肉不断增长的需求导致乌干达养猪生产的大规模扩张。这导致人与猪之间的接触增加。猪可作为新发传染病的宿主。因此，鉴定潜在的人畜共患病原体对公共卫生监测具有重要意义。在本研究中，在对非洲猪瘟进行常规监测期间，使用高通量焦磷酸测序方法筛选乌干达家猪的RNA和DNA病毒的存在。从乌干达的5个地区收集了16头家猪的血清样本，并进行了相应的汇总。提取基因组DNA和RNA，在454 GS-FLX平台上测序。在分类单元中，53%的序列指向家猪（Sus scrofa）。鉴定出非洲猪瘟病毒、转矩病毒（TTVs）和猪内源性逆转录病毒。有趣的是，两个RNA来源池（B和C）的序列显示98%的序列与恩杜姆病毒（NDUV）同源。这些序列都不属于昆虫纲，这表明这些序列不太可能是蚊子核酸污染的结果。这是首次报道家猪作为恩杜姆病毒的脊椎动物宿主。NDUV以前仅从食用蚊子中分离出来。因此，NDUV是一种潜在的人畜共患病原体，特别是考虑到人-牲畜-蚊子接触的风险日益增加。
The rising demand for pork has resulted in a massive expansion of pig production in Uganda. This has resulted in increased contact between humans and pigs. Pigs can act as reservoirs for emerging infectious diseases. Therefore identification of potential zoonotic pathogens is important for public health surveillance. In this study, during a routine general surveillance for African swine fever, domestic pigs from Uganda were screened for the presence of RNA and DNA viruses using a high-throughput pyrosequencing method. Serum samples from 16 domestic pigs were collected from five regions in Uganda and pooled accordingly. Genomic DNA and RNA were extracted and sequenced on the 454 GS-FLX platform. Among the sequences assigned to a taxon, 53% mapped to the domestic pig (Sus scrofa). African swine fever virus, Torque teno viruses (TTVs), and porcine endogenous retroviruses were identified. Interestingly, two pools (B and C) of RNA origin had sequences that showed 98% sequence identity to Ndumu virus (NDUV). None of the reads had identity to the class Insecta indicating that these sequences were unlikely to result from contamination with mosquito nucleic acids. This is the first report of the domestic pig as a vertebrate host for Ndumu virus. NDUV had been previously isolated only from culicine mosquitoes. NDUV therefore represents a potential zoonotic pathogen, particularly given the increasing risk of human-livestock-mosquito contact.