Successful PEGylation of hollow encapsulin nanoparticles from Rhodococcus erythropolis N771 without affecting their disassembly and reassembly properties
Successful PEGylation of hollow encapsulin nanoparticles from Rhodococcus erythropolis N771 without affecting their disassembly and reassembly properties
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DOI：
10.1039/c7bm00207f
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发表时间：
2017-06-01
影响因子：
6.6
通讯作者：
Murakami, Yoshihiko
中科院分区：
文献类型：
--
作者：
Sonotaki, Seiichi;Takami, Taku;Murakami, Yoshihiko
我们从红平红球菌N771中开发了中空PEG化的纳米颗粒。通过基因重组的方法构建了表面带有His标签和Lys残基的中空工程化的人胰蛋白酶纳米粒。用PEG衍生物甲氧基-PEG-SCM成功地将颗粒表面上的Lys残基PEG化。因此，我们证明了中空的PEG化的工程化的纳米颗粒可以成功地分解或重新组装，即使在存在或不存在蛋白质变性剂的PEG化后。在本研究中获得的纳米颗粒具有通过可逆可控的拆卸和重新组装将亲水性化合物并入颗粒的内部空腔中的潜力。该中空聚乙二醇化明胶纳米颗粒可用作药物载体，用于在未来的医学应用中递送亲水性生物聚合物。
We developed a hollow PEGylated encapsulin nanoparticle from Rhodococcus erythropolis N771. The hollow engineered encapsulin nanoparticles with His-Tag and Lys residues on the surface were constructed by means of genetic recombination. The Lys residues on the particle surface were successfully PEGylated with a PEG derivative, methoxy-PEG-SCM. Consequently, we demonstrated that the hollow PEGylated engineered encapsulin nanoparticle could successfully disassemble or reassemble even after PEGylation in the presence or absence of a protein denaturing agent. The nanoparticle obtained in the present study has the potential to incorporate hydrophilic compounds in the internal cavity of the particle by reversibly controllable disassembly and reassembly. The hollow PEGylated encapsulin nanoparticle can be used as a drug carrier for the delivery of hydrophilic biopolymers in future medical applications.