Volume changes induced by osmotic stress in freshly isolated rat hippocampal neurons
Volume changes induced by osmotic stress in freshly isolated rat hippocampal neurons
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DOI：
10.1007/s004240050734
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发表时间：
1998-11-01
影响因子：
4.5
通讯作者：
Wadman, WJ
中科院分区：
文献类型：
--
作者：
Aitken, PG;Borgdorff, AJ;Wadman, WJ
渗透压改变哺乳动物中枢神经元体积的程度以前尚未确定。我们分离CA 1锥体细胞和测量细胞体积在四个不同的方式。通过省略不同量的NaCl降低细胞外渗透压摩尔浓度（pi（o）），并通过添加甘露醇升高;测试的pi（o）极值范围为134至396 mosm/kg。当pi（o）降低时，细胞肿胀变化很大。我们区分了三种类型的细胞，根据他们的反应：“生产细胞”，其体积立即开始增加;“延迟反应细胞”，在2分钟或更长的潜伏期后肿胀;和“耐药细胞”，其体积在暴露于低渗溶液中没有变化。当pi（o）升高时，大多数细胞缓慢收缩，在15-20分钟内达到最小体积。我们既没有观察到调节体积减少也没有增加。我们的结论是，海马CA 1区锥体神经元的膜的水渗透性是低的相比，其他类型的细胞。细胞骨架对质膜的机械支持可能有助于抵抗肿胀并保护神经元免受肿胀诱导的损伤。
The degree to which osmotic stress changes the volume of mammalian central neurons has not previously been determined. We isolated CA1 pyramidal cells and measured cell volume in four different ways. Extracellular osmolarity (pi(o)) was lowered by omitting varying amounts of NaCl and raised by adding mannitol; the extremes of pi(o) tested ranged from 134 to 396 mosm/kg. When pi(o) was reduced, cell swelling varied widely. We distinguished three types of cells according to their response: "yielding cells" whose volume began to increase immediately; "delayed response cells" which swelled after a latent period of 2 min or more; and "resistant cells" whose volume did not change during exposure to hypoosmotic solution. When pi(o) was raised, most cells shrank slowly, reaching minimal volume in 15-20 min. We observed neither a regulatory volume decrease nor an increase. We conclude that the water permeability of the membrane of hippocampal CA1 pyramidal neurons is low compared to that of other cell types. The mechanical support of the plasma membrane given by the cytoskeleton may contribute to the resistance to swelling and protect neurons against swelling-induced damage.