Glycine and GABAA receptors suppressively regulate the inspiratory-related calcium rise in the thoracic inspiratory cells of the neonatal rat.
Glycine and GABAA receptors suppressively regulate the inspiratory-related calcium rise in the thoracic inspiratory cells of the neonatal rat.
复制标题
甘氨酸和GABAA受体抑制了新生大鼠胸腔吸气细胞中与灵感相关的钙升高。
DOI：
10.1186/s12576-022-00850-4
复制
发表时间：
2022-10-03
影响因子：
2.3
通讯作者：
Izumizaki, Masahiko
中科院分区：
文献类型：
--
作者：
Mikami, Yoshihiro;Iizuka, Makito;Onimaru, Hiroshi;Izumizaki, Masahiko
我们以前证明，在一个孤立的脑干脊髓制备新生大鼠，局部浴应用士的宁（广泛的甘氨酸和GABAA受体拮抗剂）脊髓增强胸部吸气运动活动。在此，为了研究提供兴奋性输入的运动神经元的吸气脊髓中间神经元的参与，我们使用该制剂进行钙成像。将荧光钙指示剂俄勒冈州绿色488 BAPTA-1 AM注射到胸髓的腹内侧表面。在显示吸气相关荧光变化>基线荧光强度的2%的所有细胞中，当焦点深度加深时，吸气相关荧光变化降低。应用士的宁脊髓增加了吸气相关的细胞内钙上升，在这些细胞。这些结果表明，吸气中间神经元活动的增强可能参与了吸气运动活动的增强。
We previously demonstrated that in an isolated brainstem–spinal cord preparation from neonatal rats, a local bath application of strychnine (a broad antagonist of glycine and GABAA receptors) to the spinal cord enhances thoracic inspiratory motor activity. Herein, to investigate the involvement of the inspiratory spinal interneurons that provide excitatory input to the motoneuron, we conducted calcium imaging using this preparation. Oregon Green 488 BAPTA-1 AM, a fluorescent calcium indicator, was injected into the ventromedial surface of the thoracic cord. In all cells that showed inspiratory-related fluorescence changes > 2% of the baseline fluorescence intensity, the inspiratory-related fluorescence change decreased when the focal depth was deepened. The application of strychnine to the spinal cord increased the inspiratory-related intracellular calcium rise in these cells. These results suggest that the enhancement of inspiratory interneuron activity could be involved in this enhancement of inspiratory motor activity.
登录
查看更多内容
影响因子：
2.6
作者：
Iizuka M;Ikeda K;Onimaru H;Izumizaki M
通讯作者：
Izumizaki M
影响因子：
4
作者：
Onimaru H;Yazawa I;Takeda K;Fukushi I;Okada Y
通讯作者：
Okada Y
影响因子：
3.3
作者：
Brockhaus, J;Ballanyi, K
通讯作者：
Ballanyi, K
影响因子：
5.5
作者：
Iizuka, M
通讯作者：
Iizuka, M
影响因子：
5.3
作者：
Ruangkittisakul, Araya;Schwarzacher, Stephan W.;Ballanyi, Klaus
通讯作者：
Ballanyi, Klaus