Impact of CD34 subsets on engraftment kinetics in allogeneic peripheral blood stem cell transplantation
Impact of CD34 subsets on engraftment kinetics in allogeneic peripheral blood stem cell transplantation
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DOI：
10.1038/sj.bmt.1704755
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发表时间：
2005-01-01
影响因子：
4.8
通讯作者：
Sayed, D
中科院分区：
文献类型：
--
作者：
Kamel, AM;El-Sharkawy, N;Sayed, D
我们的目的是评估，可能是第一次，CD 34亚群对异基因外周血干细胞移植（PBSCT）的植入动力学的影响。分析62例PBSC移植物成分的总CD 34+绝对计数/kg和以下亚群：DR-和+、CD 71 +/-、CD 38 +/-、CD 33 +/-和CD 61 +/-。报告了至ANC >0.5且>1 x 10（9）/l和血小板>20且>50 x 10（9）/l的时间。使用每个参数的中值来区分快速和缓慢植入。CD 34 +/ DR -（P = 0.002）、CD 34 +/ 38-（P = 0.02）、CD 34 +/CD 61-（P = 0.04）和总CD 34+细胞量（P = 0.04）对中性粒细胞早期植入有显著的预测能力。CD 34 +/CD 61+（P = 0.02）、CD 34 +/CD 38-和总CD 34+细胞数（P = 0.04）、CD 34 +/CD 71-（P = 0.05）4个参数对早期血小板植入有显著预测力。比较接受>和接受<阈值剂量的患者，只有CD 34 +/CD 38-失去了中性粒细胞植入的意义;只有CD 34 +/CD 61+保留了血小板植入的意义（P = 0.03）;此外，前一组需要的血小板输注量显著减少（P = 0.018）。我们得出结论，在同种异体PBSCT中，早期中性粒细胞植入的最佳预测因子是绝对CD 34 +/ DR-，而早期血小板植入的最佳预测因子是绝对CD 34 +/CD 61+细胞剂量。
Our objective was to evaluate, probably for the first time, the impact of CD34 subsets on engraftment kinetics in allogeneic PBSC transplantation (PBSCT). PBSC graft components were analyzed in 62 cases for the absolute count/kg of total CD34+ and the following subsets: DR- and +, CD71+/-, CD38+/-, CD33+/- and CD61+/-. Time to ANC >0.5 and >1 x 10(9)/l and platelets >20 and >50 x 10(9)/l was reported. The median value for each parameter was used to discriminate rapid from slow engraftment. Four parameters showed significant predictive power of early neutrophil engraftment, namely CD34+/ DR - ( P = 0.002), CD34+/ 38- ( P = 0.02), CD34+/ CD61- ( P = 0.04) and total CD34+ cell dose ( P = 0.04). Four parameters showed significant predictive power of early platelet engraftment, namely CD34+/ CD61+ ( P = 0.02), CD34+/ CD38 - and total CD34+ cell dose (P = 0.04) and CD34+/ CD71 - ( P = 0.05). Comparing patients who received >to those who received < the threshold dose(s), only CD34+/ CD38- lost its significance for neutrophil engraftment; and only CD34+/CD61+ retained its significance for platelet engraftment ( P = 0.03); furthermore, the former group required significantly fewer platelet transfusions ( P = 0.018). We concluded that in allogeneic PBSCT, the best predictor of early neutrophil engraftment is the absolute CD34+/ DR- and for early platelet engraftment is the absolute CD34+/ CD61+ cell dose.