HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS
HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS
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DOI：
10.1016/s0021-9673(00)96972-3
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发表时间：
1990-01-19
期刊：
影响因子：
--
通讯作者：
ALPERT, AJ
中科院分区：
文献类型：
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作者：
ALPERT, AJ
当亲水色谱柱用疏水（主要是有机）移动的相洗脱时，保留随溶质的亲水性而增加。正相色谱法的这种变体被提议称为双作用色谱法。这种色谱法具有普遍的实用性。蛋白质、肽、氨基酸、寡核苷酸和碳水化合物的混合物都被解析，其选择性与其他模式的选择性互补。通常，洗脱顺序与反相色谱法相反。研制了一种亲水性中性填料，用于高效毛细管相互作用色谱。亲水相互作用色谱法特别适用于处理组蛋白、膜蛋白、磷酸化氨基酸和肽等复杂的溶质。亲水相互作用色谱分离类似于通过分配机制获得的那些。特别是DNA的层析，类似于在基于聚乙二醇和葡聚糖溶液的含水两相系统中观察到的分配。
When a hydrophilic chromatography column is eluted with a hydrophobic (mostly organic) mobile phase, retention increases with hydrophilicity of solutes. The term hydrophilic-interaction chromatography is proposed for this variant of normal-phase chromatography. This mode of chromatography is of general utility. Mixtures of proteins, peptides, amino acids, oligonucleotides, and carbohydrates are all resolved, with selectivity complementary to those of other modes. Typically, the order of elution is the opposite of that obtained with reversed-phase chromatography. A hydrophilic, neutral packing was developed for use in high-performance hydrophilic-interaction chromatography. Hydrophilic-interaction chromatography is particularly promising for such troublesome solutes as histones, membrane proteins, and phosphorylated amino acids and peptides. Hydrophilic-interaction chromatography fractionations resemble those obtained through partitioning mechanisms. The chromatography of DNA, in particular, resembles the partitioning observed with aqueous two-phase systems based on polyethylene glycol and dextran solutions.