IDENTIFICATION AND CHARACTERIZATION OF A SUPPRESSOR ELEMENT IN THE 5'-FLANKING REGION OF THE MOUSE ANDROGEN RECEPTOR GENE
IDENTIFICATION AND CHARACTERIZATION OF A SUPPRESSOR ELEMENT IN THE 5'-FLANKING REGION OF THE MOUSE ANDROGEN RECEPTOR GENE
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DOI：
10.1093/nar/22.18.3693
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发表时间：
1994-09-11
影响因子：
14.9
通讯作者：
TINDALL, DJ
中科院分区：
文献类型：
--
作者：
KUMAR, MV;JONES, EA;TINDALL, DJ
雄激素通过雄激素受体（雄激素受体）在男性生殖器官的发育和维持中起重要作用。为了从分子水平研究AR的调控机制，我们从小鼠雄激素受体（mAR）基因5′侧区分离出一个1571 bp的片段并进行了测序。将克隆的5'缺失构建体转染到含有氯霉素乙酰转移酶（CAT）基因的载体中，发现在-146 ~ +131序列上存在一个启动子。这些实验还表明存在一种抑制因子。进一步的表征表明，抑制子存在于-486至-351之间。它在天然AR启动子和异源胸苷激酶启动子的背景下起作用。转染-546/+131构建体，其中假定的抑制元件（-421至-448）被删除，导致基础CAT活性增加，这表明抑制因子仅限于小鼠AR基因5'侧区域的28bp元件。
Androgens play an important role in the development and maintenance of male reproductive organs through the androgen receptor (AR). In order to study the mechanism of regulation of AR at the molecular level, a 1571 bp fragment in the 5'-flanking region of the mouse androgen receptor(mAR) gene was isolated and sequenced. Transfection of 5'-deletion constructs cloned into vectors containing the chloramphenicol acetyl transferase (CAT) gene indicated the presence of a promoter in the sequence -146 to +131. These experiments also suggested the presence of a suppressor element. Further characterization indicated that the suppressor is present between -486 to -351. It is functional in the context of the natural AR promoter and the heterologous thymidine kinase promoter. Transfection of a -546/+131 construct in which the putative suppressor element (-421 to -448) had been deleted caused increased basal CAT activity suggesting that the suppressor is limited to this 28 bp element in the 5'-flanking region of the mouse AR gene.