Genetic diversity and carriage dynamics of Neisseria lactamica in infants
Genetic diversity and carriage dynamics of Neisseria lactamica in infants
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DOI：
10.1128/iai.73.4.2424-2432.2005
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发表时间：
2005-04-01
影响因子：
3.1
通讯作者：
Maiden, MCJ
中科院分区：
文献类型：
--
作者：
Bennett, JS;Griffiths, DT;Maiden, MCJ
内酰胺奈瑟菌是一种主要在婴儿上呼吸道发现的无害人类病原体，与脑膜炎奈瑟菌密切相关，脑膜炎奈瑟菌是一种具有全球意义的病原体。定殖N.当脑膜炎球菌携带率较低时，内酰胺可能是儿童期发生的脑膜炎球菌病免疫力增强的原因。这一观察导致了N.内酰胺全细胞或组分是新型脑膜炎球菌疫苗的潜在成分。然而，N.对儿童中的内酰胺菌了解甚少，这给选择用于疫苗开发的代表性分离株带来了困难。这一问题通过N.来自两项婴儿细菌携带纵向研究的内酰胺分离物。研究包括100和216名受试者，N。分别从4周龄至96周龄和从2周龄至24周龄监测内酰胺携带。在56周龄时观察到的最大携带率为44%，大多数携带者（75例中的54例，72%）在多次访视时获得分离株。了N.从75名婴儿中分离出69种不同的基因型。携带通常是长期的，在收购后的28周内，平均损失率低于每周1%。在研究过程中，75名婴儿中只有11名携带一种以上的基因型独特分离株。一些参与者与兄弟姐妹共享相同的分离株，但没有人与父母共享相同的分离株。这些发现对基于这种生物体的疫苗设计具有影响。
Neisseria lactamica, a harmless human commensal found predominantly in the upper respiratory tracts of infants, is closely related to Neisseria meningitidis, a pathogen of global significance. Colonization with N. lactamica may be responsible for the increase in immunity to meningococcal disease that occurs during childhood, when rates of meningococcal carriage are low. This observation has led to the suggestion that N. lactamica whole cells or components are potential constituents of novel meningococcal vaccines. However, the dynamics of carriage and population diversity of N. lactamica in children are poorly understood, presenting difficulties for the choice of representative isolates for use in vaccine development. This problem was addressed by the multilocus sequence typing of N. lactamica isolates from two longitudinal studies of bacterial carriage in infants. The studies comprised 100 and 216 subjects, with N. lactamica carriage monitored from age 4 weeks until age 96 weeks and from age 2 weeks until age 24 weeks, respectively. The maximum observed carriage rate was 44% at 56 weeks of age, with isolates obtained on multiple visits for the majority (54 of 75, 72%) of carriers. The N. lactamica isolates were genetically diverse, with 69 distinct genotypes recovered from the 75 infants. Carriage was generally long-lived, with an average rate of loss of under 1% per week during the 28 weeks following acquisition. Only 11 of the 75 infants carried more than one genotypically unique isolate during the course of the study. Some participants shared identical isolates with siblings, but none shared identical isolates with their parents. These findings have implications for the design of vaccines based on this organism.